降糖中药1号对胰岛细胞分泌功能影响的体外实验

背景在前期研究基础上对降糖中药1号进行了其对离体胰岛细胞促胰岛素分泌的作用试验.目的探讨降糖中药1号的作用机制,为临床实验提供科学依据.设计取新生猪胰岛细胞进行传代培养,观察降糖1号对体外培养的胰岛细胞分泌胰岛素的影响.单位山东省立医院病理科及山东大学山东省立医院神经内科.材料实验于2004-02在山东省医学科学院中药药理研究室完成,选用美国长白杜络克种的新生猪.方法获得新生猪胰岛细胞,随机分为5组,每组3瓶,培养液组加入等量的培养液,优降糖组加入4 nmol/L的优降糖,降糖1号0.2 mL,0.15 mL,0.1 mL组分别加入降糖1号滤液0.2 mL,0.15 mL,0.1 mL,各组均放入培养箱中培养孵育3 h后取上清液,用放射免疫法测定胰岛素含量.主要观察指标各组胰岛细胞活性成分的比较.结果在基础培养液中的各组胰岛素水平差异不显著.但降糖1号0.2 mL,0.15 mL和0.1 mL组胰岛素释放量均比基础培养液胰岛素含量增加(784.72±81.25),(462.25±14.91);(711.73±91.46),(467.62±15.56);(679.40±78.84),(475.82±13.33)mU/L],与加入中药剂量的倍比关系不明显.优降糖组刺激胰岛素分泌显著增加(857.96±77.08),(470.58±15.43)mU/L].结论降糖中药1号能够刺激胰岛细胞,使胰岛素分泌量显著增加,从而有良好的降糖作用.

Experiment in effect of jiangtang zhongyao No.1 on islet cell secretion in vitro

BACKGROUND: On bases of preceding study on jiangtang zhongyao No.1 (glucose-reducing No. 1), the experiment was performed in its effect on promotion of insulin secretion of islet cells in vitro.OBJECTIVE: To explore the mechanism of glucose-reducing No.1 so as to provide the scientific evidence for clinical experiment.DESIGN: Neonatal porcine islet cells were collected for generative culture to observe the effect of glucose-reducing No.1 on insulin secretion of islet cells cultured in vitro.SETTING: Department of Pathology of Shandong Province Hospital and Neurological Department of Shangdong Province Hospital of Shandong University.MATERIALS: The experiment was performed in Research Room of Chinese Herbal Pharmacology in Shandong Academy of Medical Sciences, in which, American neonatal pig was selected as the object.METHODS: The obtained neonatal porcine islet cells were randomized into 5 groups, 3 bottles in each group. In culture solution group, the culture solution of equal volume was added. In daonil group, daonil 4 nmol/L was added and in glucose-reducing No.1 0.2 mL, 0.15 mL and 0.1 mL groups, the filter solution of glucose-reducing No.1 0.2 mL, 0.15 mL and 0.1 mL were added successively. The samples in each group were incubated in culture box for 3 hours, and then the upper clear solution was collected to assay insulin content with radioimmunoassay (RIA).MAIN OUTCOME MEASURES: Comparison of active components of islet cells in each group.RESULTS: In basic culture solution, insulin levels were not different significantly among groups. The release amounts in glucose-reducing No. 10.2 mL, 0.15 mL and 0.1 mL groups were increased compared with insulin content in basic culture solution (784.72±81.25), (462.25 ±14.91);(711.73±91.46), (467.62±15.56); (679.40±78.84), (475.82±13.33) mU/L],and multiple proportion relationship was not obvious with dosage of Chinese herb. In daonil group, insulin secretion was increased significantly (857.96±77.08), (470.58±15.43) mU/L].CONCLUSION: Jiangtang zhongyao No.1 simulates islet cells to increase significantly the secreted amount of insulin so that the glucose is well reduced.