沙眼衣原体重组口服活疫苗的体液免疫学特性

目的检测所构建的沙眼衣原体(Ct)重组疫苗株口服免疫小鼠后，产生的体液免疫应答。方法将所构建的重组菌以5×108菌落形成单位(CFU)/只的剂量，口服免疫雌性Balb/c小鼠，随机分为7组，每组3只。于免疫后第2，7，14，21，28，35和42d各取1组，采集血清、小肠肠腔冲洗液和阴道冲洗液标本。分别以D型Ct和鼠伤寒杆菌临床分离株为抗原，检测各组血清、小肠冲洗液和阴道冲洗液标本中特异性IgG，IgA和IgM。取抗D型Ct血清IgG阳性标本和阴道冲洗液IgA阳性标本，分别用于检测与C型Ct的交叉反应。结果在血清标本中，有抗D型Ct和抗鼠伤寒杆菌特异性IgG和IgM，在小肠冲洗液和阴道冲洗液标本中有抗上述两种抗原特异性IgG和IgA。抗D型CtIgG阳性血清标本和IgA阳性阴道冲洗液标本与C型Ct均有交叉反应。结论所构建的疫苗株免疫小鼠后，能够诱导Ct特异性IgG及IgA产生。

Immunological properties of a recombinant oral live vaccine against Chlamydia trachomatis

Aim To investigate the antibody responses of the mice immunizedwith recombinant vaccine against Chlamydia trachomatis. Methods Groups of three mice were sacrificed on 2, 7, 14, 21, 28, 35 and 42 days after oral adminstration with 5× 108 CFU of the recombinant vaccine strain per mouse. Serum IgG and IgM, as well as IgG and IgA in small intestinal washing and vaginal washing, were detected against both C. trachomatis serovar D and S. typhimurium isolate. Cross reaction to C. trachomatis serovar C were also detected in IgG-positive serum and IgA-positive vaginal washing. Results Specific IgG was detectable in serum of mice immunized with the vaccine strain, while IgA was detectable in mucosal washing. Conclusion The vaccine strain previously constructed was capable of inducing specific serum IgG and secretory IgA against C. trachomatis.