| Bmi-1基因及其编码蛋白在SKM-1细胞系中的表达 |
| |
| 引用本文： | 马国光,郭坤元,尚振川.Bmi-1基因及其编码蛋白在SKM-1细胞系中的表达[J].肿瘤防治研究,2006,31(2):83-85. |
| |
| 作者姓名： | 马国光 郭坤元 尚振川 |
| |
| 作者单位： | 南方医科大学附属珠江医院血液科; |
| |
| 摘 要： | 目的探讨人类Blymphoma Mo-MLVinsertion region(Bmi-1)基因及其编码蛋白在骨髓增生异常综合征(myelodysplasia syndrome,MDS)发病中的地位和作用。方法以正常骨髓和白血病细胞系K562为对照,利用半定量RT-PCR和Western Blot研究MDS细胞系SKM-1中Bmi-1基因在mRNA和蛋白水平的改变。结果SKM-1中Bmi-1在mRNA和蛋白水平均较正常组织有明显表达(P<0.05),二者表达水平与K562细胞系无显著差异(P>0.05)。结论Bmi-1基因在MDS亚型RAEB细胞系SKM-1中有明显表达,其在RAEB发病中可能具有重要意义。
|
| 关 键 词： | Bmi-1 MDS SKM-1 |
| 文章编号： | 1000-8578（2006）02-0083-03 |
| 收稿时间： | 03 21 2005 12:00AM |
| 修稿时间： | 2005-03-212005-06-24 |
A Study on the Expression of Bmi-1 in SKM-1 Cell Line in vitro |
| |
| MA Guo-guang,GUO Kun-yuan,SHANG Zhen-chuan.A Study on the Expression of Bmi-1 in SKM-1 Cell Line in vitro[J].Cancer Research on Prevention and Treatment,2006,31(2):83-85. |
| |
| Authors: | MA Guo-guang GUO Kun-yuan SHANG Zhen-chuan |
| |
| Institution: | Department of Hematology; Zhujiang Hospital Affiliated of Nanfang Medical University; Guangzhou 510018; China; |
| |
| Abstract: | Objective To determine the role of Bmi-1 gene and its coded protein in the incidence of myelodysplasia syndrome. Methods To determine the expression of Bmi-1on the level of mRNA and protein in MI3S cell line SKM-1 by means of semi-quantities RT-PCR and Western Blot technique, normal bone marrow and cell line K562 as negative and positive comparison specifically. Results Bmi-1expression level SKM-1 differs from that of normal bone marrow remarkably, while the difference is not remarkable from that of K562. Conclusion Bmi-1 may play an important role in the incidence of MDS subtype RAEB. |
| |
| Keywords: | Bmi-1 MDS SKM-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《肿瘤防治研究》浏览原始摘要信息 |
| 点击此处可从《肿瘤防治研究》下载免费的PDF全文 |
