| 山茱萸组织培养技术的研究 |
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| 引用本文： | 薛建平,张爱民,王月辉,盛玮. 山茱萸组织培养技术的研究[J]. 中国中药杂志, 2003, 28(2): 118-121 |
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| 作者姓名： | 薛建平 张爱民 王月辉 盛玮 |
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| 作者单位： | 淮北煤炭师范学院,生物系,安徽,淮北,235000 |
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| 基金项目： | 安徽省教育厅重点项目(2000JL193ZD) |
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| 摘 要： | 目的:研究山茱萸茎段组织培养技术,为山茱萸优良株系的快繁建立最佳条件。方法:选择山茱萸优良母株的茎段为外植体,分别在不同的培养基中培养,形成大量丛芽,再诱导单株苗生根成为完整植株。结果与结论:茎段在WPM+6-BA2.0mg·L-1+ZT0.1mg·L-1+NAA0.1mg·L-1培养基上,能形成大量丛芽;在WPM+6-BA1.0mg·L-1+ZT0.1mg·L-1+GA30.5mg·L-1+NAA0.1mg·L-1中继代培养,可获大量壮苗;切取单株苗在1/2 MS+6-BA0.1mg·L-1+IBA1.0 mg·L-1培养基中能生根,移栽并成活。
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| 关 键 词： | 山茱萸 茎段 植物组织培养 |
| 文章编号： | 1001-5302(2003)02-0118-04 |
| 收稿时间： | 2002-05-20 |
| 修稿时间： | 2002-05-20 |
Study on Plant Tissue Culture of Cornus officinalis |
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| XUE Jian-ping;ZHANG Ai-min;WANG Yue-hui;SHENG Wei. Study on Plant Tissue Culture of Cornus officinalis[J]. China Journal of Chinese Materia Medica, 2003, 28(2): 118-121 |
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| Authors: | XUE Jian-ping ZHANG Ai-min WANG Yue-hui SHENG Wei |
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| Affiliation: | Department of Biology, Huaibei Coal Industry Teacher's College, Huaibei 235000, Anhui, China. xuejp2000@yahoo.com.cn |
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| Abstract: | To set up the optimums of tissue culture for Cornus officinalis. Method: A section of stems of the fine varieties of Cornus officinalis were strictly sterilized, cultivated and geminated in medium with addition of different kinds of hormone. Result and Conclusion: The WPM medium with 6-BA 2.0 mg·L-1+ZT0.1 mg·L-1+NAA 0.1mg·L-1 was the optimum medium for the multiplication of adventitious buds. The WPM medium with 6-BA 1.0 mg·L-1+ZT0. 1 mg·L-1+GA3 0.5 mg·L-1+NAA 0.1mg·L-1 was the optimum medium for cultivation in strength. The plantlet could root well in 1/2MS medium with IBA1.0mg·L-1+6-BA0.1mg·L-1. |
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| Keywords: | Cornus officinalis stem plant tissue culture |
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