环孢菌素A治疗对再生障碍性贫血患者骨髓细胞凋亡的影响

目的 探讨环孢菌素A（CsA)治疗对再生障碍性贫血（再障）患者骨髓单个核细胞Fas（CD95）抗原表达及凋亡的影响作用。方法 用流式细胞术检测骨髓（BM）CD34^ 细胞、CD34^-细胞Fas抗原的表达，用原位末端标记法（TUNEL）测定骨髓单个核细胞凋亡率。结果 应用CsA治疗再障2个月时的总有效率为40％。再障患者骨髓CD34^ 细胞百分率显著低于正常对照（P＜0．05），再障患者骨髓CD34^ 细胞Fas表达率显著高于正常对照（P＜0．01），用CsA治疗后，再障患者骨髓单个核细胞凋亡率显著低于治疗前（P＜0．01）。CsA治疗前CD34^ 细胞Fas表达率与骨髓单个核细胞凋亡率呈正相关。结论 CsA治疗可降低再障患者骨髓单个核细胞凋亡率，CD34^ 细胞Fas的异常表达与再障的发病有关。

Apoptosis of bone marrow cells aplastic anemia patients treated with cyclosporin A

Objective To evaluate the effects of cyclosporin A (CsA) on the apoptosis of bone marrow cells of aplastic anemia (AA) patients. Methods The apoptosis of and Fas antigen expression on the bone marrow mononuclear cells (MNC) in 25 AA patients and 10 normal controls were assayed by TUNEL and FACS, respectively. Result The percentage of CD 34 cells was decreased significantly in AA patients than in normal controls (P<0 05), and so did the percentage of CD 34 Fas cells (P<0.05). The percentage of apoptotic cells in AA patients was higher than that of controls (P<0.01). After the patients were treated with CsA for 2 months, the percentage of apoptotic cells was decreased (P<0.01). The percentage of CD 34 Fas cells was positively correlated with that of apoptotic cells in AA patients. Conclusion Fas is involved in the apoptosis of CD 34 cells of AA patients. CsA could reduce the percentage of apoptosis of bone marrow cells in AA patients.