坐骨神经损伤与修复过程中相应脊髓神经元变化的形态学观察

目的探讨坐骨神经损伤与再生修复过程中相应脊髓前角运动神经元的形态学变化和嗅被膜细胞(OECs)对神经元的保护作用。方法采用硅胶管套接切断的大鼠坐骨神经实验模型,将30只大鼠随机分为两组,治疗组硅胶管内注射OECs悬液,对照组注射生理盐水(SAL),应用尼氏法对术后7d、14d、30dSAL组与OECs组脊髓前角运动神经元进行形态学观察,比较两组同类神经元的存活率。结果OECs组治疗侧脊髓前角外侧核大、中型运动神经元的形态结构和存活率与对照组有明显差别。术后7d,OECs组治疗侧神经元数目较SAL组伤侧丢失减少,存活率上升。术后14d和30d,OECs组治疗侧神经元数目较SAL组伤侧明显增加,存活率明显升高,大多数神经元轮廓清楚,尼氏体清晰。结论OECs能减少坐骨神经损伤后相应脊髓前角运动神经元的退行性变。

The morphological observation of related spinal motorneurons changes after sciatic nerve injury and repair in rats

Objective To explore the morphological changes of related spinal anerior horn motorneurons after injury and repair of rats sciatic nerve and protective effect of OECs.Methods The rats with the left sciatic nerve transected were used as models. Thirty rats were randomized into a treated group (OECs was injected into the silica gel tube) and a control group (saline was injected). The morphological changes of related spinal motorneurons at 7,14 and 30 days after sciatic nerve operation was observed by Nissl, the percentage of survived motorneurons in two groups was compared.Results There were significant differences in the morphological changes and survive rate of related spinal motorneurons between the treated groups and the control groups after operation. Compared with the control groups,the numbers of spinal motorneurons was increased and the percentage of the survived spinal motorneurons was rised after operation of 7days;the above changes were more obviously after operation of 14 days and 30 days,the Nissl bodies in more neurons were clear.Conclusion OECs could protect the degenerated spinal motorneurons after sciatic nerve transected.