雾化吸入BCG对哮喘小鼠气道炎性作用的影响

目的探讨卡介苗（BCG）雾化吸入对变应性哮喘的治疗作用及其与支气管肺泡灌洗液（BALF）中γ-干扰素（IFN-γ）的关系。方法昆明小鼠32只，随机分成三组，正常对照组，哮喘模型组及治疗组。治疗组又分为BCG治疗组和地塞米松治疗组。治疗组与哮喘模型组一起制作哮喘模型。观察小鼠气道壁炎症变化，并收集支气管肺泡灌洗液（BALF），测定BALF中细胞总数，嗜酸性粒细胞和淋巴细胞数，用酶联免疫法测定BALF中IFN-γ的含量。结果哮喘模型组BALF中细胞总数和嗜酸细胞数显著高于正常对照组（P〈0．01），BALF中IFN-γ水平显著低于正常组（P〈0．01）；BCG治疗组、地塞米松治疗组BALF中细胞数、EOS细胞数显著低于哮喘组，而IFN-γ水平显著高于哮喘组（P〈0．01）。BCG治疗组各项指标与地塞米松治疗组相比差异无统计学意义（P〉0．05）。结论雾化吸入BCG能明显抑制哮喘小鼠气道的炎性反应，吸入BCG对哮喘小鼠有治疗作用。

The impact of bacilli calmette-guerin vaccine fog-inhalation on airway inflammation in asthmatic mice

Objective To study the therapy effect of boris-Bacillus Calmette-Guérin (BCG) fog-inha-lation on allergic asthma in mice and the relation with IFN-γ, levels in BALF. Methods 40 Kunming mices were randomly divided into three groups: normal group, asthmatic model group, preventive group. The preventive group was randomly divided three groups again :presensitization BCG preventive group,challenging BCG preven-tive group and dexamethasone preventive group. The preventive group after deal, asthmatic model group were made in allergic asthma model in OVA. Pathological scores, bronchoalveolar lavage(BAL) total cell count anddifferentiation were studied. IFN-γ,levels in BAL fluid (BALF) were detected by ELISA. Results The number of BALF total cell count and eosinophils in asthmatic model group were more than in the normal group (P <0.01),IFN-γ level in BALF was decreased(P<0.01);In presensitization BCG prevention group and dexam-ethasone prevention group the number of BALF total cell count and eosinophils were significantly lower than asthma group, and the IFN-γ level significantly higer than asthma group (P<0.01-0.05). These parameters in challenging BCG prevention group were not significantly changing compared with asthmatic model group (P>0.05). Conclusion BCG fog-inhalation can not only markedly inhibit but also treat the airway inflammation in asthmatic mice.