TT病毒粪口传播途径的初步研究

目的 初步探讨TT病毒血源性传播以外的传播途径。方法 用半巢式聚合酶链反应检测6位TT病毒感染的肝病患者粪便标本中TTV DNA，测序，分型，比较其与血清中病毒滴度的关系。 结果 在血清病毒滴度较高的2例患者（10^4/ml,10^3/ml)的粪便标本中检出了TTV DNA（10^4ml,10^2ml），基因型分别为1a,1b；在血清病毒滴度仅为10^1/ml的4位患者中，仅1例检出TTV DNA（10^1/ml)，为一种新的基因型。结论 肝病患者粪便标本中TTV病毒颗粒含量与血清滴度直接相关，TT病毒可能具有血源及粪口双重传播途径。

A preliminary study on fecel-oral transmision of TT virus

Objective\ To study the fecel-oral and blood transfusion of TT virus. Methods \ Paired feces and serum samples from 6 patients with type B and/or C hepatitis were tested for TTV DNA and its titers by PCR with seminested primers.Genotypes were determined after their sequences were compared with the original N22 and TA278 clone.Results\ TTV DNA was detected in sera from all patients,while it was detected in feces from 3 patients,including 2 with high viral titers in serum.The detection of fecal TTV DNA was dependent on the viral titers in serum.TTV isolates in 3 pairs of feces and serum had identical sequence of 222 base pairs.Their genotypes were 1a,1b and 2,respectively.Conclusion\ The excretion of TTV into feces indicates that TTV would be transmitted not only parenterally but also nonparenterally by a fecal oral route.