核酸扩增检测技术在血液筛查中的应用 |
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引用本文: | 孙振秀,许雷,杨忠思,刘晓华. 核酸扩增检测技术在血液筛查中的应用[J]. 中国卫生质量管理, 2012, 19(4): 78-80 |
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作者姓名: | 孙振秀 许雷 杨忠思 刘晓华 |
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作者单位: | 青岛市中心血站 山东青岛266071 |
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摘 要: | 从建立PCR标准化实验室,制定标准操作规程;强化人员培训;正确选择设备设施,做好校准、维护与保养;选择合格的实验耗材;制定严格的防污措施,做好环境质控;做好检测环节质控;确保记录完整等方面,总结了核酸扩增检测技术在血液筛查中的应用实践.
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关 键 词: | 核酸扩增检测技术 聚合酶链式反应(PCR) 实验室 |
The Application of Nucleic Acid Amplification Test Technology in Blood Screening |
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Affiliation: | SUN Zhenxiu,XU Lei,YANG Zhongsi,et al.Qingdao Blood Center,Qingdao,Shandong,266071,China |
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Abstract: | The paper summed up the application practice of NAT in blood screening from the following aspects: the establishment of the PCR standardized laboratory to develop standard operating procedures;strengthening personnel training;the correct choice of equipment and facilities,and to make its calibration and maintenance;the selection of qualified laboratory supplies;formulation of strict anti-pollution measures,good environmental quality control;performing test quality control;ensuring the integrity of the record,etc. |
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Keywords: | Nucleic Acid Amplification Test Technology Polymerase Chain Reaction(PCR) Laboratory |
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