1－烯丙基氯－3诱导神经细胞内游离钙和游离钠离子改变

用鸡胚脑神经细胞对１－烯丙基氯－３引起的细胞内游离Ｃａ~（２＋）、游离Ｎａ~＋的变化进行了研究。结果显示，在１－烯丙基氯－３的作用下，神经细胞内游离Ｃａ~（２＋）、游离Ｎａ~＋浓度随毒物浓度的增加而明显增高，呈显著的剂量－效应关系。细胞内游离Ｎａ~＋的改变在低剂量组无Ｃａ~（２＋）变化明显，细胞外高钾能使细胞内游离钙增加，但对细胞内游离Ｎａ~＋作用不明显。提示１－烯丙基氯－３引起的中毒性周围神经病可能与神经细胞内Ｎａ~＋、Ｃａ~（２＋）增加有关。

ALLYL CHLORIDE-INDUCED CHANGES IN CYTOSOLIC IONIZED CALCIUM AND SODIUM OF CHICKEN BRAIN NERVE CELLS

The present paper used fluorescence molecular probe Fura- 2 /AM and SBFI/AM, determined the changes of cytosolic ionized calcium ( Ca2+ ] i) and cytosolic ionized sodium ( Na+ ] i) in 14 day chicken embryon whole brain nerve cells. The results showed: Ca2+] i increased sharply with allyl chloride concentrations increased. The doseresponse relationship was obvious and had definite statistical significance (P<0.01, n= 4) . Na+] i also added with allyl chloride concentrations increased. The statistical significance had marked difference (P<0.01, n=4) except the low dose of 40.72 mmol/L. The results can reveal that electrophysiological changes caused by allyl chloride may relate with Na+ and Ca2+ increased inside nerve cells.