HPLC法测定田基黄中槲皮素-7-O-α-L-鼠李糖苷的含量

目的建立田基黄中槲皮素-7-O-α-L-鼠李糖苷的含量测定方法,为田基黄药材的质量控制和综合开发利用提供一定的科学依据。方法采用HPLC法。色谱柱为Hypersil C18柱(250 mm×4.6 mm,5μm),流动相为体积分数0.04%的乙腈-磷酸水(体积比77∶23),流速为1.0 mL.min-1,检测波长为371 nm,柱温为35℃。结果槲皮素-7-O-α-L-鼠李糖苷质量浓度在3.12~99.84 mg.L-1(r=0.9998,n=6)内与峰面积呈良好的线性关系;平均回收率为99.1%(RSD=1.5%,n=9)。结论该方法简便、快速、精密度好,可用于田基黄的质量控制。

Content determination of quercetin-7-O-a-L-rham-noside in Hypericum japonicum by HPLC

Abstract: objective To establish an HPLC method for the determination of quercetin-7-O-α-L-rhamnoside in Hypericum japonicum. Method The separation was performed on an Hypersil C₁₈ column (250 mm × 4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile-0.04% aqueous phosphoric acid （V:V=23:77）at the flow rate of 1.0 mL·min-1. The detection wavelength was set at 371 nm and the column temperature was set at 35 ℃. Result The calibration curve was linear over the range of 3.12-99.84 mg·L-1 for linarin (r = 0.999 8). The average recoveries were 99.1% (RSD = 1.5%, n = 9). Conclusions The method is simple