仙茅苯甲酸酯类酚苷对去卵巢骨质疏松大鼠的作用

目的：评价仙茅苯甲酸酯类酚苷（benzylbenzoate glucosides from Curculigo orchioides，COBG）对去卵巢大鼠的抗骨质疏松作用。方法：70只3月龄的Sprague-Dawley大鼠被随机分为正常对照组和去卵巢组。再将去卵巢大鼠分为6组，连续12周分别灌胃给予蒸馏水，1mg／kg尼尔雌醇，6、18和54mg／kgCOBG，3．0g／kg仙茅乙醇提取物。用双能X线吸收仪测定骨密度，并制备胫骨切片进行骨形态计量分析，采用试剂盒测定血清和尿的生化指标。结果：去卵巢大鼠骨质流失和骨组织结构退化显著，其血清碱性磷酸酶活性升高，尿钙排出增加，血清抗氧化物水平降低（P〈0．05，P〈0．01〉。6、18和54mg／kgCOBG使去卵巢大鼠骨密度显著增加，骨组织微结构得到改善，超氧化物歧化酶和谷胱甘肽过氧化物酶的损耗减少，并抑制氧化产物丙二醛的增加，减少尿钙排出（P〈0．05，P〈0．01）。结论：COBG通过改善去卵巢大鼠的抗氧化状态防止骨丢失，COBG有可能成为防治绝经后骨质疏松的有效药物。

Antiosteoporotic effects of benzylbenzoate glucosides from Curculigo orchioides in ovariectomized rats

OBJECTIVE： To evaluate the antiosteoporotic orchioides （COBG） in ovariectomized （OVX） effects of benzylbenzoate glucosides from Curculigo rats. METHODS： A total of 70 female Sprague-Dawley rats were assigned to sham-operated and OVX model groups. The OVX rats were further divided into six subgroups treated by gavage with vehicle, 1 mg/kg of nylestriol, 6, 18 and 54 mg/kg of COBG and 3.0 g/kg of ethanol extract of Curculigo orchioides respectively for 12 weeks. Bone mineral density （BMD） was measured by dual-energy X-ray absorptiometry. The sections of tibia were prepared for histomorphometric analysis. The biomarkers in serum and urine were determined using reagent kits. RESULTS： Ovariectomy induced the bone loss with the activities of increased serum alkaline and microarchitectural deterioration of bone tissue phosphatase and loss of calcium through the excretion in urine, and decreased levels of antioxidant in serum （P〈0. 05, P〈0. 01）. Administration of 6, 18 and 54 mg/kg of COBG significantly increased the BMD, improved the microarchitecture of bone tissue, prevented the depletion of antioxidant enzymes like superoxide dismutase and glutathione peroxidase, inhibited the Lncrease of malondialdehyde in serum and reduced the excretion of urine calcium in OVX rats （P〈0.05, P〈0.01）. CONCLUSION： COBG could prevent the bone loss through improving the antioxidant status, which offers a potential new therapeutic drug for postmenopausal osteoporosis.