黄豆提取物的雌激素依赖作用对戊四唑诱发的大鼠癫痫的影响

目的：研究黄豆提取物对经戊四唑（pentylenetrazole，PTZ）诱发癫病发作的女性荷尔蒙缺失雌性大鼠与正常雌性大鼠的不同作用，以及因性别差异引起的植物雌性激素对行为的影响。方法：雄性Wistar大鼠被随机分为雄性生理盐水组，雄性低、中、高剂量黄豆提取物治疗组，每组8只；雌性Wistar大鼠随机分为假手术生理盐水组，假手术低、中、高剂量黄豆提取物治疗组，去卵巢生理盐水组，去卵巢低、中、高剂量黄豆提取物治疗组，每组8只。去卵巢大鼠在氯胺酮麻醉下行卵巢切除术。分别给予各组大鼠生理盐水及不同剂量黄豆提取物治疗2周后腹腔内注射戊四唑。将大鼠放置在树脂玻璃笼内，记录最小阵挛性癫痫发作（minialclonicseizure，MCS）潜伏期和强直性阵挛性癫痫发作（generalizedtonic-clonicseizure，GTCS）潜伏期。结果：与雄性生理盐水组大鼠比较，雄性低、中剂量黄豆提取物治疗组的MSC和GTCS潜伏期显著缩短（P〈10．05或P〈0．01）。雌性假手术大鼠在给予黄豆提取物治疗后，其MSC和GTCS潜伏期没有显著改变。与去卵巢生理盐水组比较，去卵巢低、高剂量黄豆提取物治疗组的MSC和GTCS潜伏期明显缩短（P〈0．05或P〈0．01）。结论：黄豆的植物雌激素能影响由PTZ诱发的癫痫发作的轻重程度，但其影响程度与卵巢激素水平有关。机制还有待进一步研究。

Estrogen-dependent effect of soy extract on pentylenetetrazole-induced seizures in rats

OBJECTIVE： To study the different effects of soy extract on pentylenetetrazole （PTZ）-induced seizures in the presence and absence of ovarian hormones in rats, and the gender-dependent differences in the effects of phytoestrogens on behavior. METHODS： Male and female Wistar rats were randomly divided into nine groups with eight in each, namely, male-saline （M-saline）, male-low-dose soy （M-LDS）, male-high-dose soy （M-HDS）, sham-saline （Sh-saline）, sham-low-dose soy （Sh-LDS）, sham-high-dose soy （Sh-HDS）, ovariectomized- saline （OVX-saline）, ovariectomized-low-dose soy （OVX-LDS） and ovariectomized-high-dose soy （OVX-HDS）. The rats of groups 7 to 9 were ovariectomized under ketamine anesthesia. The rats of groups 2, 5 and 8 were treated by 20 mg/kg of soy extract while the animals of groups 3, 6 and 9 received 60 mg/kg of soy extract for two weeks. In groups 1, 4 and 7, saline was injected instead of soy extract. The animals were then injected by a single dose of PTZ （90 mg/kg body weight, intraperitoneally） and placed in a plexiglas cage and the latency to minimal clonic seizure （MCS） and generalized tonic-clonic seizure （GTCS） was recorded. RESULTS： Both MCS and GTCS latency in M-LDS and M-HDS groups was significantly lower than that in M-saline group （P〈0.05 or P〈0.01）. Treatment for female sham rats by soy extract did not affect MCS and GTCS latency. The animals of OVX-LDS and OVX-HDS groups had lower MCS and GTCS latency in comparison with OVX-saline group （P〈0.05 or P〈0.01）. CONCLUSION： It is concluded that the phytoestrogens of soy affect seizure severity induced by PTZ, but their effects are different in the presence or absence of ovarian hormones. Howev- er, further studies are necessary to be done.