HLA-DR单倍型与中国南方汉族肺结核的相关性分析

目的　探讨HLA DR基因单倍型在中国南方汉族肺结核发病机制中的可能作用。方法 采用病例 对照的研究方法 ,应用PCR SSP技术对 110例中国南方汉族肺结核患者和101例中国南方汉族健康对照者的 23个HLA DR等位基因进行分型 ,比较两组间DR53-DRB1、DR52-DRB1和DR51-DRB1单倍型频率 (HF)并计算其相对危险性(RR)。结果 DR16-DR52单倍型的频率肺结核病例组显著高于对照组 (0.01<P<0.05),其RR为3.10;DR1-DR53、DR1-DR52、DR13.3-DR53、DR12-DR53、DR13.3-DR52、DR7-DR52.DR9-DR52单倍型的频率肺结核病例组显著低于对照组（前四者P<0.01，后三者 0.01<P<0.05),其 RR分别为 0.29、0.26、0.38、0.52、0.25、0.10、0.42。结论DR12-DR53、DR7-DR52、DR9-DR52单倍型的存在可能与中国南方汉族肺结核的发病有关联，而其它单倍型的差异则可能是因其组成基因的基因频率差异所致。

Analysis of Relevance of Pulmonary Tuberculosis to HLA-DR Gene haplotype among Han Nationality of Southern Chinese

Objective To study possible role of HLA-DR gene haplotype in incidence of pulmonary tuberculosis among Han nationality of southern Chinese.Methods Case-control study was implemented and haplotype frequencies(HF) of DR53-DRB1、DR52-DRB1 and DR51-DRB1 were compared and relative risks (RR)were calculated by using PCR-SSP to detect 23 alleles of HLA-DR gene sites of 110 tuberculosis cases and 101 controls who come from Guangdong, Guangxi, Hunan, Hubei,Jiangxi and Fujian.Results Frequency of DR16-DR52 haplotype in pulmonary tuberculosis cases is strikingly higher than that in controls (0.01< P <0.05) and its RR is 3.10.Frequencies of DR1-DR53、DR1-DR52、DR13.3-DR53、DR12-DR53、DR13.3-DR52、 DR7-DR52、DR9-DR52 haplotype in pulmonary tuberculosis cases is remarkably lower than that in controls (for DR1-DR53、DR1-DR52、DR13.3-DR53、DR12-DR53, P <0.01, for DR13.3-DR52、 DR7-DR52、DR9-DR52, 0.01< P <0.05) and their RRs are 0.29、0.26、0.38、0.52、0.25、0.10 and 0.42 respectively.Conclusion DR12-DR53、DR7-DR52、DR9-DR52 haplotype are potential relative to incidence of pulmonary tuberculosis among Han nationality of southern Chinese. However, difference of frequencies of DR16-DR52、DR1-DR53、DR1-DR52、DR13.3-DR53、DR13.3-DR52 haplotype between pulmonary tuberculosis cases and controls may be caused by remarkable differences of gene frequency.