DNA定量分析检测对胸腔积液的诊断价值研究

目的 探讨细胞DNA定量分析在不明原因胸水诊断中的应用价值。方法 收集本院2015年9月~2016年3月122例胸腔积液患者抽取胸水，标本制片后分别经巴氏染色行脱落细胞学检查，Feulgen染色后行细胞DNA定量分析。结果 122例胸腔积液中有56例恶性胸腔积液，66例良性胸腔积液。细胞学检测恶性胸水敏感度为82.14%，特异性为98.48%，阳性预测值97.87%，阴性预测值86.67%。细胞DNA定量分析检测恶性胸水敏感度为80.36%，特异度为90.91%，阳性预测值88.24%，阴性预测值84.51%。两种方法诊断结果一致性一般（Kappa=0.473，P＜0.001），差异无统计学意义（P=1.0）。结论 DNA定量分析检测是一种较敏感而特异的肺癌的筛查技术，DNA异倍体有望作为良恶性胸水诊断有价值的标志物。

Quantitative Analysis of DNA for Diagnosis of Pleural Effusion

Abstract:Objective To evaluate the value of DNA quantitative analysis in the diagnosis of unexplained hydrothorax.Methods A total of 122 patients with pleural effusion were collected from our hospital in September 2015~March 2016.After taking the tablets, they were stained by PAP staining and cytological examination.After Feulgen staining, cell DNA was quantitatively analyzed.Results There were 56 cases of malignant pleural effusion and 66 cases of benign pleural effusion in 122 cases of pleural effusion.The sensitivity of malignant pleural effusion was 82.14%,the specificity was 98.48%,the positive predictive value was 97.87%,and the negative predictive value was 86.67%.The sensitivity of DNA in malignant pleural effusion was 80.36%,the specificity was 90.91%,the positive predictive value was 88.24%,and the negative predictive value was 84.51%.The results of the two methods were consistent(Kappa=0.473, P<0.001),and the difference was not statistically significant(P=1.0).Conclusion DNA quantitative analysis is a sensitive and specific screening technology for lung cancer.DNA aneuploidy is expected to be a valuable marker for the diagnosis of benign and malignant pleural effusion.