miR-3691-3p靶向调控E2F3/PRDM1对前列腺癌细胞增殖、迁移和侵袭的影响

miR-3691-3p targeted E2F3/PRDM1 inhibits the biological characteristics of prostate cancer

Objective: To investigate the effects of miR 3691 3p regulated target genes E2F3 and PRDM1 on the proliferation, migration and invasion of prostate cancer DU145 cell line. Methods: miR 3691 3p mimic with various concentrations was transfected into DU145 cell line, RT PCR and Western blotting were used to detect the expression level of E2F3, PRDM1 mRNA and protein. The small interfering RNA scrambled siRNA, E2F3 siRNA and PRDM1 siRNA were transfected into DU145 cells. The expression levels of E2F3, PRDM1 mRNA were detected by RT PCR and MTT, Transwell and scratch tests were used to detect the proliferation, migration and invasion of DU145 cell line. Results: The expression of E2F3(P<0.01) and PRDM1(P<0.05) mRNA decreased with the increase of miR 3691 3p mimic transfection concentration in DU145 cell line, the expression level of both two protein were decreased (P<0.01). Compared with the negative control group, the expression of E2F3 and PRDM1 in DU145 cell lines transfected with E2F3 siRNA and PRDM1 siRNA were significantly decreased (E2F3: t=31, P<0.01; PRDM1: t=10.44, P<0.01). The proliferation, migration and invasion of DU145 cells transfected with E2F3 siRNA, were significantly decreased (all P<0.01). The proliferation, invasion of DU145 cells transfected with PRDM1 siRNA were significantly decreased(all P<0.01), but the migration ability was not changed(P>0.05). Conclusion: The expression of miR 3691 3p is low in DU145 cells, promoting the biological characteristics of cells by targeting E2F3 and PRDM1.