免疫荧光法检测FSHR与GnRHR在胃癌组织中的分布及其共定位研究

研究卵泡刺激素受体与促性腺激素释放激素受体在胃癌组织中的定位、分布及共存性。方法 选取我院胃肠外科经手术和病理证实的48例胃癌患者的病理组织石蜡包块标本，采用免疫荧光双标记定位方法检测胃癌标本中FSHR及GnRHR的定位及分布。结果 FSHR和GnRHR在胃癌组织细胞胞浆中均有分布，免疫荧光反应阳性物质分布于细胞质，细胞核呈阴性反应，二者分布模式相同。结论 FSHR与GnRHR在胃癌组织中分布具有共存性，提示其可能对胃癌的病理发生机制具有重要的影响。

Study on the Distribution and Co-localization of FSHR and GnRHR in Gastric Cancer Tissues by Immunofluorescence Assay

Objective To investigate the distribution of follicle-stimulating hormone receptor（FSHR） and gonadotropin releasing horm- one receptor （GnRHR） colocalization with at the gastric cancer. Methods Using the immunity fluorescence double mark localization method to examine in 48 example stomach cancer specimen FSHR and the GnRHR localization and the distribution.Results FSHR and GnRHR organize in the cell cytoblastema at the stomach cancer to have the distribution,the immunity fluorescence responded the masculine material distributes in the cytoplasm， the cell nucleus assumes the negative reaction，the two distributed pattern is same. Conclusion FSHR and GnRHR organize the distribution at the stomach cancer to have the coexistence，it is suggested that it may have an important effect on the pathogenesis of gastric cancer.