LSD1对非小细胞肺癌细胞增殖与迁移的影响

目的: 探讨赖氨酸特异性组蛋白去甲基化酶1(lysine specific demethylase 1，LSD1)在非小细胞肺癌(NSCLC)中的表达及对细胞增殖与迁移的影响。方法: qRT PCR检测52例NSCLC标本和对应癌旁组织中LSD1 mRNA水平，分析其与NSCLC临床病理特征的关系。qRT PCR检测NSCLC细胞A549与正常肺支气管上皮细胞16HBE中LSD1 mRNA表达；将A549细胞分为2组，分别转染LSD1干扰RNA(si LSD1组)，阴性对照RNA(对照组)；MTT实验、克隆形成实验、流式细胞技术、Transwell迁移实验分别检测两组细胞增殖、凋亡、迁移能力的差异；qRT PCR和蛋白质印迹法分别检测细胞上皮间质转化相关分子mRNA和蛋白水平。结果： 与癌旁组织相比，NSCLC组织中LSD1 mRNA表达升高(t=2.861,P<0.05);LSD1表达量与NSCLC患者TNM分期及淋巴结转移相关(χ2=4.062，6.047，P均<0.05)，与患者年龄、性别、吸烟、肿瘤直径、病理分型无明显相关性(P>0.05)。A549细胞中LSD1 mRNA表达量明显高于16HBE细胞（t=3.569,P<0.05)。与对照组相比，si LSD1组A549细胞增殖与迁移能力降低，细胞凋亡率显著增加(t=3.618,P<0.05)，E 钙黏蛋白表达上调(t=5.607，P<0.05)，波形蛋白表达下调(t=-6.628，P<0.05)。 结论： LSD1在NSCLC组织及细胞中表达上调，可能通过促进上皮间质转化参与细胞增殖和迁移调控。

(Department of Medical Imaging,Affiliated Hospital of Jiangsu University,Zhenjiang Jiangsu 212001, China)

Objective: To investigate the expression of lysine specific demethylse 1(LSD1) in non small cell lung cancer (NSCLC) and its effect on cell proliferation and migration. Methods: The expression of LSD1 mRNA in 52 pairs of NSCLC tissues and adjacent tissues were determined by qRT PCR; and the relationship between LSD1 expression and clinical features were analyzed. Expression of LSD1 mRNA in NSCLC cell A549 and normal lung bronchial epithelial cells 16HBE were determined by qRT PCR. A549 cells were divided into 2 groups, transfected with LSD1 interfering RNA(si LSD1 group) and negative control RNA(control group), respectively. Cell proliferation, apoptosis and migration in the two groups were detected by MTT assay, clone formation assay, flow cytometry, and Transwell migration test. Epithelial mesenchymal transition related mRNA and protein expression levels were detected by qRT PCR and Western blotting. Results: Compared with adjacent tissues, LSD1 in NSCLC was significantly up regulated(t=2.861,P<0.05). The expression of LSD1 was correlated with TNM stage and lymph node metastasis in NSCLC patients (χ2=4.062, 6.047, P<0.05). There was no significant correlation between LSD1 expression and age, sex, smoking, tumor size and pathological type. Compared with the control group, si LSD group showed lower proliferation and metastasis, higher rate of cell apoptosis(t=3.618,P<0.05), upregulated expression of E cadherin(t=5.607，P<0.05) and downregulated expression of vimentin(t=-6.628，P<0.05). Conclusion: The expression of LSD1 was up regulated in NSCLC. LSD1 might promote proliferation and metastasis by regulating EMT related gene.