沉默SLP-2 可抑制人宫颈癌细胞的迁移及侵袭能力

目的研究沉默SLP-2对人宫颈癌细胞迁移及侵袭能力的影响及其作用机制。方法用siRNA转染人宫颈腺癌Hela细胞及宫颈鳞癌Siha细胞作为沉默组，同时设立空白对照组及阴性对照组，转染48 h后，用Western blot检测SLP-2蛋白的表达量，用划痕实验、Transwell 实验检测沉默SLP-2 对Hela 和Siha 细胞迁移能力的影响，用基质胶Transwell 实验检测沉默SLP-2 对Hela 和Siha 细胞侵袭能力的影响，用Western blot 检测沉默SLP-2 后Hela 和Siha 细胞上皮间质转化标志分子E-cadherin、β-catenin、vimentin及上皮间质转化上游转录因子Twist蛋白表达量的变化。结果与对照组相比，Western blot结果显示沉默组SLP-2蛋白的表达量明显下降；划痕实验显示沉默组的划痕面积愈合率明显下降（P<0.01）；Transwell实验及基质胶Transwell实验均显示沉默组穿过小室膜至下室的细胞数明显减少（P<0.01）；Western blot结果显示沉默组上皮细胞标志分子E-cadherin及β-catenin表达升高，而间质细胞标志分子vimentin表达下降，表明Hela细胞和Siha细胞的上皮间质转化受到抑制；Western blot结果显示沉默组Twist蛋白的表达量也明显下调。结论沉默SLP-2可抑制人宫颈癌细胞上皮间质转化（EMT）的发生，使宫颈癌细胞的迁移及侵袭能力下降，其作用机制可能与下调Twist蛋白的表达有关。

Silencing of SLP-2 inhibits the migration and invasion of cervical cancer cells in vitro

Objective To investigate the effect of SLP-2 silencing on the migration and invasion of human cervical cancer cellsand explore the mechanism. Methods Small interfering RNA (siRNA) was used to knockdown the expression of SLP-2 in Helacells and Siha cells. At 48 h after the transfection, the cells were examined for SLP-2 expression with Western blotting, andwound healing assay and Transwell assay were used to evaluate the changes in the cell migration; Matrigel Transwell assaywas used to evaluate the changes in the invasion ability of the cells. The expressions of E-cadherin, β-catenin, vimentin andTwist in Hela and Siha cells following the transfection were detected with Western blotting. Results Compared with thecontrol cells, siRNA transfection significantly lowered the expression of SLP-2 and suppressed the migration and invasionability of Hela cells and Siha cells (P<0.01). Silencing SLP-2 induced obvious up-regulation of epithelial cell phenotype proteinsE-cadherin and β-catenin, down- regulated the expression of interstitial cell phenotype protein vimentin, and lowered theexpression of Twist in the cells. Conclusion Silencing SLP-2 via siRNA transfection can inhibit epithelial-mesenchymaltransition of human cervical cancer cells and lower their migration and invasion abilities possibly in relation with downregulatedexpression of Twist.