长链非编码RNA MALAT-1基因的敲除抑制人喉鳞状细胞癌Hep-2细胞的增殖和迁移 |
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引用本文: | 韩跃峰,陈德尚,李慧,王晓敏,张明洁,杨洋.长链非编码RNA MALAT-1基因的敲除抑制人喉鳞状细胞癌Hep-2细胞的增殖和迁移[J].南方医科大学学报,2018,38(8):923. |
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作者姓名: | 韩跃峰 陈德尚 李慧 王晓敏 张明洁 杨洋 |
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摘 要: | 目的探讨敲除长链非编码RNA MALAT-1基因对人喉鳞状细胞癌Hep-2细胞的影响。方法使用实时聚合酶链反应(RTPCR)
以永生化鼻咽上皮(NPE)细胞株NP-69作为参照检测FaDu、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达,使用shmalat1
慢病毒转染Hep-2细胞,RT-PCR检测其MALAT1的表达。增殖速率分析,MTT法明确MALAT-1对细胞增殖的影响。流式细
胞术分析细胞周期和细胞凋亡。采用Transwell 小室检测Hep-2 细胞的迁移。最后使用Matrigel 侵袭实验评估shmalat1 和
shCtrl慢病毒转染的Hep-2细胞的侵袭能力。结果使用qPCR检测FaDu、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达,结果
发现与NP-69细胞相比,FaDu、Hep-2和鼻咽癌CNE-2Z细胞MALAT1的表达显著增加。敲除MALAT1明显抑制Hep-2细胞增
殖。与MOCK和shCtrl 细胞相比,MALAT1-shRNA 慢病毒转染细胞S期细胞数量显著增加(P<0.01)。此外,细胞在G2/M期
的百分比下降(P<0.01)。下调MALAT1时,Hep-2细胞的侵袭和迁移都受到抑制。结论MALAT1在喉鳞状细胞癌高表达。敲
除长链非编码RNA MALAT-1基因对人喉鳞状细胞癌Hep-2细胞的增殖、凋亡、侵袭和迁移起抑制作用。
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Long chain non-coding RNA MALAT-1 gene knockdown inhibits growth and migration
and promotes apoptosis of human laryngeal squamous cell carcinoma Hep-2 cells in vitro |
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Abstract: | Objective To investigate the effect of knocking down long chain non-coding RNA MALAT-1 gene on the biological
behaviors of human laryngeal squamous cell carcinoma Hep-2 cells. Method With immortalized nasopharyngeal epithelial
(NPE) cell line NP-69 as the reference, MALAT1 expression in FaDu, Hep-2 and nasopharyngeal carcinoma CNE-2Z cells were
detected using real-time PCR. Hep-2 cells were transfected with shmalat1 lentivirus and the expression of MALAT1 was
detected. MTT assay, flow cytometry, Transwell assay and M Atrigel invasiveness test were used to evaluate the effect of
MALAT-1 knockdown on the proliferation, cell cycle, cell apoptosis, migration, and invasiveness of Hep-2 cells. Results
Compared with NP-69 cells, Hep-2 cells, FaDu cells, and CNE-2Z cells all showed significantly increased MALAT-1
expression. In Hep-2 cells, knockdown of MALAT-1 significantly inhibited the cell proliferation, increased the cell percentage
in S phase (P<0.01), decreased the cell percentage in G2/M phase (P<0.01), and attenuated the migration and invasiveness of the
cells. Conclusion MALAT-1 is over-expressed in laryngeal squamous cell carcinoma, and knocking down MALAT-1 gene can
significantly suppress the proliferation, invasion and migration and promotes apoptosis of the cancer cells. |
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