TIM-3基因启动子区-1516G/TRs153538和外显子区-882C/TRs474853,-574T/GRs151746位点多态性与蒙古族儿童哮喘的关系研究

目的 探讨哮喘免疫调节基因T细胞免疫球蛋白黏蛋白域3(Tim-3)启动子区多态性位点-1516G/T(Rs10053538)和外显子区多态性位点-882C/T(Rs4704853),-574T/G(Rs1051746)与内蒙古地区蒙古族儿童哮喘的遗传易感性。方法收集赤峰市蒙医中医医院和红山区妇幼保健所2014年6月～2016年12月哮喘组儿童129例,平均年龄6.2岁; 正常体检对照组儿童130例,平均年龄6.0岁。采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测所有研究对象的三个多态性位点的基因型,进行病例对照研究分析。结果 哮喘组-574T/G(Rs1051746)位点基因多态性的T等位基因明显高于对照组,差异有统计学意义(OR=0.239,95%CI 0.067～0.858,P<0.05)。哮喘组Tim-3启动子区-1516G/T(Rs10053538)位点基因多态性与对照组比较OR=0.835,95%CI 0.371～1.883,P=0.664; 哮喘组外显子-882C/T(Rs4704853)位点基因多态性与对照组比较,差异无统计学意义(OR=0.326,95%CI0.33～3.172,P=0.310)。结论 Tim-3基因-574T/G(Rs1051746)位点与蒙古族儿童哮喘易感性相关,为内蒙古地区儿童哮喘的发病机制研究提供参考。

Relationship between TIM-3 Gene Promoter Region -1516G/T(Rs10053538),Exon Region -882C/T(Rs4704853),-574T/G(Rs1051746)Polymorphisms and Asthma of Mongolian Nationality Children

Abstract:Objective To elucidate whether the polymorphisms within the asthma immune regulator gene T cell immunoglobulin domain and mucindomain 3 two polymorphism loci,-882C/T(Rs4704853),-574T/G(Rs1051746)at exon region and one polymorphism loci,-1516G/T(Rs10053538)at promoter region were associated with the risk of Mongolian nationality childhood asthma.Methods Polymerase chain reaction(PCR)was used to test the genotypes of three polymorphism loci among 129 cases(average 6.2y)of asthma and 130 controls(average 6.0y)in a case-control study collected from June of 2014to June of 2016.Results The results of PCR-RFLP showedthat the three polymorphisms in TIM-3 gene were present in the study population.TIM-3 -574T/G(Rs1051746)polymorphism was associated with Mongolian nationality childhood asthma(OR=0.239,95%CI 0.067～0.858,P<0.05),but at promoter region -1516G/T(Rs10053538)polymorphism(OR=0.835,95%CI 0.371～1.883,P=0.664)and -882C/T(Rs4704853)at exon region(OR=0.326,95%CI 0.33～3.172,P=0.310)were not.Conclusion These results indicated that -574T/G(Rs1051746)polymorphism of the TIM-3 gene was associated with Mongolian nationality childhood asthma,providing a better understanding of the pathogenesis of the allergic asthma among children in Inner Mongolia.