四君子汤血清药效成分的HPLC-MS分析

目的: 分析四君子汤水煎液和含药血清的化学成分，探讨其发挥药效的物质基础。方法: 按照四君子汤处方中药材比例制备水煎液，灌胃6只大鼠后2 h采血，采用高效液相色谱质谱联用法鉴定水煎液和含药血清中的有效成分。结果： 四君子汤水煎液中检测出10种化学物质，其中皂苷类7种，分别为人参皂苷Rb1、Re、Rg1、Rb2、Rd、Rg3、甘草酸；黄酮类3种，分别为甘草苷、甘草素、甘草香豆素。含药血清中检测出人参皂苷Rb1、Re、甘草酸共3种化学原型成分，甘草次酸、甘草素葡萄糖醛酸共2种代谢产物。 结论： 四君子汤水煎液和含药血清中的有效成分主要来自人参及甘草，其中人参以原型化合物，而甘草则以水解产物的形式存在于在血液中。

Analysis of the major metabolites of Sijunzi decoctionin rat serum based on HPLC-MS

Objective: To determine the decoction and drug-containing serum of Sijunzi decoction by high performance liquid chromatography mass spectrometry(HPLC-MS), and analyse the major metabolites of Sijunzi decoction. Methods: The decoction was prepared according to the proportion of traditional Chinese medicinal materials of sijunzi decoction, and the blood was collected 2 h after intragastric administration Sijunzi decoction to 6 rats. HPLC-MS was used to identify the active components in decoction and drug serum. Results: According to the total ion chromatogram obtained by HPLC-MS and the fragmentation peak of mass spectrometry, it was deduced that Sijunzi decoction contained 10 chemical substances, among them, 7 saponins were ginsenoside Rb1, Re, Rg1, Rb2, Rd, Rg3, glycyrrhizic acid; 3 flavonoids were liquiritin, glycyrrhizin, licorice coumarin. Drug-containing serum contains three prototype chemical composition, which are ginsenoside Rb1, Re, glycyrrhizic acid and 2 transitional components, glycyrrhetinic acid and glycyrrhizin glucuronide. Conclusion: The chief chemical compositions of the decoction and drug-containing serum of Sijunzi decoction were ginsenosides and glycyrrhiza, the former was metabolized in blood by prototype compound while the latter was metabolized in blood by hydrolysis product.