聚乳酸-羟基乙酸共聚物载肝细胞生长因子纳米粒的构建及生物学活性评价

目的探究制备聚乳酸-羟基乙酸共聚物（PLGA）纳米粒的优化条件，构建肝细胞生长因子（HGF）纳米粒，评价其包封率、 载药量、回收率、释放度和生物学活性。方法采用复乳溶剂挥发法制备牛血清白蛋白（BSA）PLGA纳米粒，通过正交试验设计， 以粒径较小，包封率、载药量和回收率较高为考察指标，优化纳米粒的制备条件；选取优化条件制备HGF纳米粒，分别采用BCA 试剂盒和HGF-ELISA试剂盒检测BSA纳米粒和HGF纳米粒的包封率、载药量和释放度，通过CCK8增殖实验评价HGF纳米粒 的生物活性。结果优化条件下制备的HGF 纳米粒大小均匀，粒径234.4±4.8 nm，包封率（77.75±3.04）%，回收率（49.33± 9.34）%，体外释放度曲线表现为先突释，后缓释；HGF纳米粒可以促进角质形成细胞的增殖。结论复乳溶剂挥发法-优化条件 下制备的HGF纳米粒具有较高包封率，良好的缓释效果和生物学活性。

Construction and bioactivity evaluation of hepatocyte growth factor-loaded poly (lacticco- glycolic acid) nanoparticles

Objective To explore the optimum conditions for preparing poly(lactic-co-glycolic) acid (PLGA) nanoparticles and evaluate the bioactivity of hepatocyte growth factor (HGF)-loaded PLGA nanoparticles. Methods Bovine serum albumin (BSA)-loaded PLGA nanoparticles were prepared using a double emulsion-solvent evaporation method. The preparation process of nanoparticles was optimized by orthogonal test with the particle size, encapsulation efficiency (EE), drug loading (DD), and recovery as the indexes. HGF-loaded nanoparticles were then prepared under the optimized conditions. The EE, DD and release characteristics of BSA-loaded nanoparticles and HGF-loaded nanoparticles were evaluated using a BCA kit and HGF ELISA kit. The bioactivity of HGF-loaded nanoparticles was evaluated using CCK8 proliferation assay. Results The HGF-loaded nanoparticles prepared under the optimized conditions had a uniform size with a mean diameter of 234.4±4.8 nm, an EE of (77.75±3.04)% and a recovery rate of (49.33±9.34)%. The in vitro release curve highlighted an initial burst drug release followed by sustained release from the nanoparticles. HGF-loaded nanoparticles obviously promoted the proliferation of Hacat keratinocytes in vitro. Conclusion HGF-loaded nanoparticles prepared using double emulsion-solvent evaporation method under optimized conditions possesses a high EE with a good sustained drug release profile and a good bioactivity.