1例罕见B（A）血型鉴定及其临床安全输血策略初探

目的 鉴定罕见B（A）血型并探讨其临床安全输血策略。方法 利用全自动血型分析仪检测ABO血型，发现正反定型不符的一位患者，利用血型血清学和分子生物学方法对该患者及其家系中9位成员进行血型鉴定和ABO血型基因测序，分析该家族血型的遗传特点；并对该血型的患者进行临床输血探讨，确保临床输血安全。结果 患者为B（A）血型，与B101基因序列比对，均为nt640A>G，基因型鉴定为B(A)04/O01，B(A)型血清与同型红细胞以及O型、B型红细胞不凝集，B(A)型红细胞与同型以及AB型血清不凝集。结论 血型血清学方法可以对正反定型不符的B（A）血型做出初步判断，分子生物学技术可以进一步准确判断该血型；临床输血应采取自体输血、同型输注及配合相容性输血。

Study on the identification of a case with blood type B(A) and the concerned clinical safety transfusion strategies

Objective To identify the blood type B(A) which is rare to see, and to investigate the concerned clinical blood transfusion safety strategies. Methods Automatic blood analyzer was used to detect ABO blood group, and the group typing and reverse typing of a patient were found to be inconsistent. Then the blood type and gene sequencing of this patient and nine of the family members were identified by blood group serology and molecular biology to analyze the genetic characteristic of the blood group of this family and clinical transfusion research was conducted on them to ensure the safety of clinical blood transfusion. Results Which wascontrasted with B101 gene sequence were all nt640A>G and B(A) 04/O01 by gene identification.There was no agglutination between serum B(A) and O, B, B(A) red blood cells. B(A) red blood cells do not agglutinate with type B(A) and AB serum.Conclusion The group typing and reverse typing were identified as B(A) with blood group serology that could be further confirmed by molecular biological techniques; clinical blood transfusion should adopt autologous blood transfusion, same type infusion and compatible blood transfusion.