芹菜籽提取物对高尿酸血症模型大鼠的血尿酸以及抗氧化能力的影响

目的 本实验采用腺嘌呤联合乙胺丁醇诱导大鼠高血尿酸症的造模方法，观察芹菜籽提取物对高尿酸血症大鼠的降血尿酸及抗氧化作用，并进一步观察大鼠肾脏的病变。方法 雄性SD大鼠105只适应性饲养一周，随机分组，每组15只，A组（正常对照组）、B组（模型对照组）、C组（西芹3000组）、D组（别嘌醇组）、E组（低剂量组）、F组（中剂量组）、G组（高剂量组）。模型组及各给药组分别给予腺嘌呤100 mg/（kg·d）+盐酸乙胺丁醇300 mg/（kg·d）于造成高尿酸动物模型，每天于造模后4 h给予治疗药物。C组给予西芹3000片，24 mg/100g体重，每100 g给予0.5 ml体积灌胃；D组给予别嘌醇2.4 mg/100g；D、F、G组分别给予芹菜籽提取物70、140、280 mg/100g（E组灌胃按照560 mg/100g体重灌胃一周后改为70 mg/100g体重芹菜籽提取物溶液）。模型组和对照组分别给予等体积的蒸馏水灌胃。大鼠每周记录体重。实验的第三周结束后，禁食12 h，取腹主脉血，检测肾功能指标、血清SOD、T-AOC活性和MDA水平以及肾脏病理切片。结果 造模过程中大鼠体重明显降低，药物治疗对大鼠体重无明显改善。模型组大鼠肾指数明显增加，药物治疗组队肾脏指数无明显改善，但是芹菜籽提取物中剂量的肾脏指数明显增加。模型组大鼠尿酸明显增加，肾功能明显降低，芹菜籽提取物低剂量和高剂量均能明显降低尿酸水平，提高大鼠肾脏功能。模型组大鼠血清超氧化物歧化酶活性明显降低（P＜0.001），西芹片和芹菜籽提取物可明显增加SOD活性。模型组大鼠总抗氧化能力明显增加，药物治疗对总抗氧化能力无明显影响。结论 芹菜籽提取物有明显降低尿酸的作用，以高剂量为优，且降尿酸效果和别嘌呤醇相似，优于西芹3000片；芹菜籽提取物对高尿酸大鼠肾功能有明显保护作用；芹菜籽提取物有明显抗氧化作用，表现为降低MDA和增加SOD，对总抗氧化作用不明显。

Effects of Celery Seed Extract on Serum Uric Acid and Antioxidant Capacity of Hyperuricemia Rats

Abstract:Objective By using adenine combined with ethambutanol to induce hyperuricemia in rats,the effects of celery seed extract on serum uric acid and antioxidation in hyperuricemia rats were observed.Methods 105 male SD rats were fed adaptively for one week and were randomly divided into two groups.There were 15 rats in each group,group A(normal control group),group B(model control group),group C(3000 group of celery),group D(allopurinol group),E group(low dose group),F group(middle dose group),G group (high dose group).The model group and each administration group were given adenine 100 mg/ (kg.d)+ethambutol 300 mg/(kg.d)to cause high uric acid animal model.Each day was given 4 h after making the model.Group C was given 3000 celery tablets,24 mg/100g weight and 0.5 ml volume per 100 g were given to gastric lavage;group D was given allopurinol 2.4 mg/100g;D,F,G group were given celery seed extracts 70,140,280 mg/100g respectively(E group gavage after 560 mg/100g weight to 70 mg/100g weight celery seed extract solution).The model group and the control group were given the same volume of distilled water to gavage the stomach.The rats recorded weight per week.At the end of the third week of the experiment,fasting for 12 h,taking the main vein blood of the abdomen, detecting the renal function index,serum SOD,T-AOC activity and MDA level,and pathological section of the kidney.Results The body weight of the rats was significantly reduced during the modeling process,and the drug treatment did not significantly improve the body weight of the rats.The kidney index of the model group was significantly increased,and the kidney index of the drug treatment group was not significantly improved,but the kidney index of the dose of celery seed extract was significantly increased.In the model group,the uric acid increased significantly and the renal function was significantly reduced.The low dose and high dose of celery seed extract could significantly reduce the uric acid level and improve the kidney function of rats.Serum superoxide dismutase activity wassignificantly decreased in the model group(P<0.001),and the celery tablets and celery seed extracts significantly increased SOD activity.The total antioxidant capacity of the model group was significantly increased,and the drug treatment had no significant effect on the total antioxidant capacity.Conclusion The celery seed extract has a significant effect on reducing uric acid.It is superior in high dose,and the effect of reducing uric acid is similar to that of allopurinol.It is superior to celery 3000 tablets.The celery seed extract has obvious protective effect on the renal function of rats with high uric acid;the celery seed extract has obvious anti-oxidation effect,which is shown to reduce MDA and increase SOD,and the total antioxidant effect is not obvious.