芪参二莲汤对肝纤维化大鼠基质金属蛋白酶-1及抑制因子的影响

目的 研究不同剂量芪参二莲汤对肝纤维化大鼠基质金属蛋白酶-1及抑制因子变化的影响。方法 于2017年6月~8月选择90只SD雄性大鼠作为研究对象，按照体质量随机分为6组，每组15只，其中1组为正常组，其余5组均为肝纤维化模型，将其分为模型组、秋水仙碱组以及芪参二莲汤低剂量组（5 g/kg）、中剂量组（10 g/kg）和高剂量组（15 g/kg）；各给药组分别给予相应的药物灌胃，正常组、模型组则给予生理盐水灌胃；对比各组大鼠MMP-1和TIMP-1水平以及肝纤维化指标水平。结果 正常组与模型组比较，后者的MMP-1、MMP-1/TIMP-1水平明显降低，而TLISA-1水平则明显升高，差异有统计学意义（P＜0.05）；模型组与各给药组比较，各给药组的MMP-1、MMP-1/TIMP-1水平较模型组升高，而TIMP-1水平则较模型组降低，差异有统计学意义（P＜0.05），且给药组中，以芪参二莲汤高剂量组的变化水平最为显著，差异有统计学意义（P＜0.05）。结论 15 g/kg高剂量的芪参二莲汤用于治疗肝纤维化大鼠，能够有效促进MMP-1表达、减少TIMP-1表达，进而起到逆转肝纤维化的作用。

Effects of Qishenerlian Decoction on Matrix Metalloproteinase-1 and Its Inhibitory Factors in Rats with Hepatic Fibrosis

Abstract:Objective To study the effects of different doses of Qishenerlian Decoction on matrix metalloproteinase-1 and inhibitor of hepatic fibrosis in rats.Methods In June to August 2017, 90 male SD rats were selected as the research subjects.According to the body mass,they were randomly divided into 6 groups,with 15 rats in each group,of which 1 was the normal group and the other 5 groups were liver fibrosis models.They were divided into model group,colchicine group and low dose group of Qishenerlian Decoction (5g/kg),medium dose group(10g/kg)and high dose group(15g/kg).Each drug-administered group was given the corresponding drugs,and the normal group and the model group were given normal saline.The levels of MMP-1 and TIMP-1 and liver fibrosis were compared between the groups.Results Compared with the normal group,the level of MMP-1 and MMP-1/TIMP-1 in the model group was significantly decreased,while the level of TLISA-1 was significantly higher,the difference was statistically significant(P<0.05);Compared with the drug-administered group,the MMP-1 and MMP-1/TIMP-1 levels in the model group were higher than those in the model group,while the TIMP-1 level was lower than that in the model group,the difference was statistically significant(P<0.05),and in the drug-administered group,the change level of the high-dose group of Qishenerlian Decoction was the most significant,and the difference was statistically significant(P<0.05).Conclusion The high dose Qishenerlian Decoction for 15g/kg can effectively promote the expression of MMP-1,reduce the expression of TIMP-1 and reverse the hepatic fibrosis in rats.