标记滞留技术检测小鼠子宫内膜干细胞

目的：研究小鼠子宫内标记滞留细胞的存在及其分布特点。方法选择出生3天的雌性昆明白小乳鼠，实验组乳鼠皮下注射BrdU 50μg／g，对照组皮下注射生理盐水100μl，每天2次，共注射3d。于最后一次注射后2h、1w、2w、4w和8w处死并取子宫固定，石蜡包埋用于免疫组化检测，每个时间段5只乳鼠。结果实验组标记后2h，小鼠腺体和基质中标记滞留细胞（LRCs）表达最高，分别为45．1％和57．4％，随着时间的增加，LRCs逐渐降低，到第8w在腺体中仅有极少量细胞表达，而基质细胞约有1．5％的LRCs，主要位于血管周围和内膜基层连接处。不同时相腺体和基质细胞中LRCs差异具有显著性（P〈0．05）。结论昆明白小鼠子宫内LRCs主要位于血管周围和内膜基层连接处等，这些LRCs可能为子宫内膜干细胞。

The existence of endometrial stem cell in mouse uterus

Objective： To study the existence of endometrial stem cell and its distribution in mouse uterus. Methods ： Selected female Kunming white mice after boru three days, subcutaneous injected BrdU 50ug/g in experiment group and 0. 9% NS in control group twice daily and totally injected 3 d. Put mice death after injected 2h, tw, 2w, 4w 和 8w, fixed the uterus for immunohistochemistry and pathology, 5. mice for each phase. Results： The expression of LRCs in stroma and gland cells after 2h were 45.1% and 57.4% respectively and which were gradually degraded, Only few LRCs in the gland bottom in 8w and 1.5% LRCs in stroma cells mainly located around the blood vessel and endometrial - myometrial junction. LRCs in different phases of stroma and gland cells were significance （ P 〈 0. 05 ）. Conclusion： There were endometrial stem cells in the uterus of Kunming white mice and mainly located around the blood vessel and endometrial - myometrial junction.