宫颈分泌物HPV16,18型的FQ-PCR检测对育龄夫妇生殖健康的意义

目的探讨人乳头瘤病毒（HPV）16,18亚型在河北省衡水市育龄妇女中的流行情况以及感染妇女的男性性伴侣的感染率。方法采用荧光定量PCR（FQ-PCR）技术对367名育龄妇女进行了HPV16,18检测。观察HPV16,18感染的年龄分布特点。随后,30名HPV16,18阳性妇女和30名阴性妇女的男性性伴侣被邀请进行了HPV16,18检测。结果本地区HPV16,18现患率为10.4%（38/367）。感染妇女其男性伴侣感染率为13.3%（4/30）,与对照组非感染妇女的男性伴侣的感染率0（0/30）相比,有显著性差异（P=0.019,单侧检验）。结论367例育龄妇女分泌物HPV16,18检测阳性率为10.4%,男性性伴侣的感染率为13.3%。

Detection of human papillomavirus-16 and 18 in female secretions of 367 women and the infection rate in male sex partners of infected women

Objective： To investigate the prevalence of human papillomavirus （HPV） - 16, 18 in women, and to evaluate the infection rate in male sex partners of infected women, in Hengshui City, Hebei Province, China. Methods： A total of 367 HPV -16, 18 free or unknown women attended the clinical test in Harrison International Peace Hospital, Hengshui, Hebei Province. Fluorogenic quantitative PCR （FQ- PCR） was used for the detection of HPV- 16, 18. Then 30 HPV- 16, 18 infected and 30 healthy womens male sex partners were invited to the hospital for HPV - 16, 18 test. Results： Of the 367 women, 38 women were positive for HPV - 16/18 （ 10. 4% ）. The infection rate of HPV - 16, 18 was 13. 3% （4/30） in infected women＇s male sex partners, and it is0 （0/30） in healthy women＇ s male sex partners. There was a significant difference （ P =0. 019）. Conclusion： HPV - 16, 18 DNA should be required for cervical cancer screening. Meanwhile occult infection of men should not be overlooked.