柴胡皂甙d上调人急性早幼粒白血病细胞糖皮质激素受体mRNA对细胞生长的影响

目的：观察柴胡皂甙ｄ（ＳＳｄ）对人急性早幼粒白血症细胞（ＨＬ６０）糖皮质激素受体（ＧＲ）ｍＲＮＡ表达的调节作用及对细胞生长的影响。方法：用从柴胡中提取的单体成分ＳＳｄ，以＾３Ｈ－胸腺嘧啶（＾３Ｈ－ＴｄＲ）掺入法和流式细胞仪观察细胞生长，用Ｎｏｒｔｈｅｒｎ ｂｌｏｔ分析ＳＳｄ对ＧＲ ｍＲＮＡ的改变。结果：经ＳＳｄ作用后，ＨＬ６０细胞＾３Ｔ－ＴｄＲ掺入率明显降低，呈时间和剂量依赖关系，ＳＳｄ１０μｇ／

Effect of Saikosaponin-d on Up-regulating GR mRNA Expression and Inhibiting Cell Growth in Human Leukemia Cells

OBJECTIVE: To study the effect of Saikosaponin-d (SSd) on the expression of glucocorticoid receptor (GR) mRNA and cell growth in HL60 cells. METHODS: Antiproliferation effects of SSd on HL60 cells were determined by 3H-thymidine incorporation. Cell cycle analysis was also performed by flow cytometry. GRmRNA was analyzed by northern blot analysis. RESULTS: After 48 hours treatment with 10 micrograms/ml SSd, 3H-thymidine incorporation decreased in HL60 cells and the effect was time and dose dependent. The flow cytometry analysis showed HL60 cells were arrested at G0/G1 phase. The expression of GR mRNA increased. CONCLUSION: SSd exhibits the inhibition effects on cell growth in HL60 cells and could up-regulate the expression of GR mRNA in HL60 cells.