| 柴胡皂甙d上调人急性早幼粒白血病细胞糖皮质激素受体mRNA对细胞生长的影响 |
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| 引用本文： | 步世忠,许金廉,孙继虎,陈宜张,商权.柴胡皂甙d上调人急性早幼粒白血病细胞糖皮质激素受体mRNA对细胞生长的影响[J].中国中西医结合杂志,2000,20(5):350-352. |
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| 作者姓名： | 步世忠 许金廉 孙继虎 陈宜张 商权 |
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| 作者单位： | 1. 第二军医大学生理教研室,上海,200433
2. 中国科学院上海生化研究所分子生物学国家重点实验室 |
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| 基金项目： | 国家中医药管理局基金资助(No.96F016) |
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| 摘 要： | 目的：观察柴胡皂甙ｄ（ＳＳｄ）对人急性早幼粒白血症细胞（ＨＬ６０）糖皮质激素受体（ＧＲ）ｍＲＮＡ表达的调节作用及对细胞生长的影响。方法：用从柴胡中提取的单体成分ＳＳｄ，以＾３Ｈ－胸腺嘧啶（＾３Ｈ－ＴｄＲ）掺入法和流式细胞仪观察细胞生长，用Ｎｏｒｔｈｅｒｎ ｂｌｏｔ分析ＳＳｄ对ＧＲ ｍＲＮＡ的改变。结果：经ＳＳｄ作用后，ＨＬ６０细胞＾３Ｔ－ＴｄＲ掺入率明显降低，呈时间和剂量依赖关系，ＳＳｄ１０μｇ／
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| 关 键 词： | 白血病 HL60 柴胡皂甙d 糖皮质激素受体 mRNA |
| 修稿时间： | 1999年6月12日 |
Effect of Saikosaponin-d on Up-regulating GR mRNA Expression and Inhibiting Cell Growth in Human Leukemia Cells |
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| Authors: | Bu Shizhong XU Jinlian SUN Jihu |
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| Institution: | Physiology Department, Second Military Medical University, Shanghai (200433). |
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| Abstract: | OBJECTIVE: To study the effect of Saikosaponin-d (SSd) on the expression of glucocorticoid receptor (GR) mRNA and cell growth in HL60 cells. METHODS: Antiproliferation effects of SSd on HL60 cells were determined by 3H-thymidine incorporation. Cell cycle analysis was also performed by flow cytometry. GRmRNA was analyzed by northern blot analysis. RESULTS: After 48 hours treatment with 10 micrograms/ml SSd, 3H-thymidine incorporation decreased in HL60 cells and the effect was time and dose dependent. The flow cytometry analysis showed HL60 cells were arrested at G0/G1 phase. The expression of GR mRNA increased. CONCLUSION: SSd exhibits the inhibition effects on cell growth in HL60 cells and could up-regulate the expression of GR mRNA in HL60 cells. |
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| Keywords: | Saikosaponin-d glucocorticoid receptor mRNA cell growth human leukemia cells |
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