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| 应用PCR-SSCP银染检测膀胱癌中H-ras基因突变 | |
| 引用本文： | 许绍斌,郭萍,陶玉芬,李泽惠,杨昭庆,褚嘉佑. 应用PCR-SSCP银染检测膀胱癌中H-ras基因突变[J]. 中华肿瘤防治杂志, 2002, 9(2): 151-153 |
| 作者姓名： | 许绍斌 郭萍 陶玉芬 李泽惠 杨昭庆 褚嘉佑 |
| 作者单位： | 1. 中国医学科学院中国协和医科大学医学生物学研究所,云南,昆明,650118 2. 昆明医学院病理教研室,云南,昆明,650031 3. 昆明医学院附属第二人民医院泌尿外科,云南,昆明,650101 |
| 基金项目： | 云南省科委应用基础研究基金资助 ( 95C0 71M ) |
| 摘 要： | 目的 :了解膀胱癌中H ras基因突变情况。方法 :应用PCR SSCP银染和DNA测序技术对 31例膀胱癌组织进行检测。结果 :H ras基因突变发生率为 38 7% ,突变位点在H ras基因的第 1外显子的第 12位密码子 ,由GGC变为GTC。结论 :H ras基因突变与膀胱癌的发生发展存在着一定的联系 ,H ras基因突变可望作为膀胱癌早期临床诊断有价值的指标。 |
| 关 键 词： | 膀胱肿瘤 H-ras 突变 PCR-SSCP |
| 文章编号： | 1009-4571(2002)02-0151-03 |
| 修稿时间： | 2001-11-21 |
Detection of H-ras Gene Mutation in Bladder Carcinoma by PCR-SSCP Siliver Staining |
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| XU Shao-bin,GUO Ping,TAO Yu-fen,et al.. Detection of H-ras Gene Mutation in Bladder Carcinoma by PCR-SSCP Siliver Staining[J]. Chinese Journal of Cancer Prevention and Treatment, 2002, 9(2): 151-153 | |
| Authors: | XU Shao-bin GUO Ping TAO Yu-fen et al. |
| Affiliation: | XU Shao-bin,GUO Ping,TAO Yu-fen,et al.Institute of Medical Biology,Chinese Academy of Medical Sciences and Peking Union Medicine College,Kunming 650118,China |
| Abstract: | Objective To investigate the mutations of oncogene H ras in bladder carcinoma.Methods 31 cases of bladder carcinoma were detected by PCR SSCP silver staining and DNA sequencing.Results The mutation ratio of exon 1 of H ras was 38 7%(12 cases)and the mutation site existed at the twelfth codon that changed GGC into GTC.The amino acid changed glycin into valine.The mutation of the exon 2 of H ras was not found.Conclusions The results indicated that the mutations of H ras were related with the genesis of bladder carcinomas;The H ras mutation expected to be as useful indicators for diagnoses in early bladder cancer. |
| Keywords: | bladder neoplasms H ras mutation PCR SSCP |
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