| 肝癌相关抗原kineetin基因重组蛋白致敏的树突状细胞对T细胞增殖活化的影响 |
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| 引用本文： | 赵飞兰,晁耐霞,薛慧琴,吴春林,林永达,罗彬,陈芳,谢小薰,罗国容,LUO Guo-rong. 肝癌相关抗原kineetin基因重组蛋白致敏的树突状细胞对T细胞增殖活化的影响[J]. 山东医药, 2010, 50(2): 21-23 |
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| 作者姓名： | 赵飞兰 晁耐霞 薛慧琴 吴春林 林永达 罗彬 陈芳 谢小薰 罗国容 LUO Guo-rong |
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| 作者单位： | 广西医科大学,南宁,530021 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金，广西教育厅普通课题，广西高校博士研究生创新课题 |
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| 摘 要： | 目的观察肝癌相关抗原kineetin基因重组蛋白（kinectln—MBP）致敏的树突状细胞（DC）对T细胞增殖活化能力的影响。方法从正常人外周血中分离单个核细胞，用rhGM—CSF、rhIL-4联合诱导扩增DC；用kinectin-MBP（kinectin—MBP组）、麦芽糖结合蛋白（MBP组）分别致敏DC，对照组不处理。采用ELISA法检测细胞上清液中的IL-12、IFN-γ，MTT法检测各组DC刺激自体T淋巴细胞增殖的能力。结果kinectin—MBP组上清液中IL-12、IFN-γ含量分别为（615．32±7．93）、（544．28±7．17）pg／ml，MBP组分别为（382．13±5．21）、（308．46±6．44）pg／ml对照组组分别为（299．40±10．22）、（228．03±6．70）pg/ml，三组相比，P均〈0．05。kinectin—MBP组T淋巴细胞增殖指数为53．14±0．62，MBP组为22．14±0．31，对照组为10．86±0．65，三组相比，P均〈0．001。结论体外诱导扩增的DC经kinectin-MBP致敏后具有很强的刺激自体T细胞增殖的能力。
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| 关 键 词： | 树突状细胞 kinectin基因 重组蛋白 T细胞 麦芽糖结合蛋白 |
Effect of DC sensitized with kinectin proteinon on T cell proliferation activity |
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| ZHAO Fei-lan,CHAO Nai-xia,XUE Hui-qin,WU Chun-lin,HUANG Jian,LIN Yong-da,LUO Bin,CHEN Fang,XIE Xiao-xun,LUO Guo-rong. Effect of DC sensitized with kinectin proteinon on T cell proliferation activity[J]. Shandong Medical Journal, 2010, 50(2): 21-23 |
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| Authors: | ZHAO Fei-lan CHAO Nai-xia XUE Hui-qin WU Chun-lin HUANG Jian LIN Yong-da LUO Bin CHEN Fang XIE Xiao-xun LUO Guo-rong |
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| Affiliation: | ZHAO Fei-lan,CHAO Nai-xia,XUE Hui-qin,WU Chunlin,HUANG Jian,LIN Yong-da,LUO Bin,CHEN Fang,XIE Xiao-xun,LUO Guo-rong (Guangxi Medical University,Nanning 530021,P.R.China) |
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| Abstract: | Objective To observe the effect of dendritic cells(DC) senitized with recombinant protein kinectin sensitized on T cell proliferation activity.Methods The DC was induced and expanded from normal human peripheral blood mononuclear cells(PBMCs) in a culture medium supplemented with rhGM-CSF and IL-4.For identifying the cultured DC,light and electronic microscopes were used to examine the morphologic characteristics of cultured cells.Cultured DC were impulsed by maltose binding protein(MBP) and Kinectin-MBP fu... |
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| Keywords: | dendritic cells kinectin gene recombinant protein T lymphocyte maltose binding protein |
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