RNA干扰对前列腺癌PC-3细胞血管内皮生长因子表达的研究

目的:构建前列腺癌血管内皮生长因子(VEGF)RNA干扰(RNAi)真核表达载体,并研究RNAi对前列腺癌VEGF表达的影响。方法:合成VEGFRNAi片段,将RNAi片段定向克隆于RNAi表达载体,测序鉴定;RNAi表达载体转染人前列腺癌PC-3细胞,Western印迹法检测VEGFRNAi表达载体对VEGF蛋白表达的影响,MTT法检测转染VEGFRNAi表达载体对细胞生长的抑制作用。结果:成功构建VEGFRNAi真核表达载体;VEGFRNAi组VEGF蛋白的表达明显降低,显著低于空载体组和未转染的对照组;前列腺癌PC-3细胞24、48、72h的生长抑制率分别为23.5%、33.5%、40.8%。结论:VEGFRNAi可以抑制前列腺癌PC-3细胞蛋白的表达和抑制细胞生长,为前列腺癌的生物治疗提供一定的实验基础。

RNA Interference Inhibits VEGF Expression and Growth of PC-3 in Prostate Carcinoma

Objective: To construct a eukaryotic expression vector carrying human VEGF RNAi and to study the effect of RNA interference on VEGF expression in prostate carcinoma. Methods: VEGF RNAi was synthesized, inserted into the RNA interference eukaryotic expression vector, and confirmed by the reault sequencing. The vector was transfected into prostate cancer PC-3, the VEGF expression detected by Western blot and the cell inhibiting rate determined by MTT. Results: The VEGF RNAi eukaryotic expression vector was successfully constructed. Compared with the empty vector group and the control group, the amount of VEGF protein expression was obviously decreased in the VEGF RNAi group. The inhibiting rates were 23.5 %, 33.5 % and 40.8 % at 24, 48 and 72 h respectively. Conclusion: VEGF RNAi can inhibit the protein expression and growth of PC-3, which provides an experimental base for the biological therapy of prostate cancer.