HPLC与紫外-可见分光光度计对养阴通脑颗粒有效部位的质量控制

目的:利用紫外-可见分光光度计(UV-VIS)检测养阴通脑颗粒中有效部位总黄酮含量。并利用梯度洗脱,建立高效液相色谱法(HPLC)测定养阴通脑颗粒中总黄酮有效部位中葛根素、芒柄花素、毛蕊异黄酮含量的方法。方法:以芦丁为对照品UV-VIS测定养阴通脑颗粒有效部位中总黄酮含量。采用HPLC,色谱柱为EclipseXDB-C18(150mm×4.6mm,5μm),流动相为乙腈(A)和0.04%磷酸水溶液(B)梯度洗脱,流速1.00mL/min,检测波长250nm。结果:养阴通脑颗粒有效部位总黄酮含量70%以上,葛根素的线性范围为0.530-296.300mg/L(r=0.9998),平均回收率为99.80%,RSD=0.40%,毛蕊异黄酮的线性范围为0.064-44.440mg/L(r=0.9994),平均回收率为99.70%,RSD=1.10%,芒柄花素的线性范围为0.064-43.110mg/L(r=0.9994),平均回收率为99.40%,RSD=1.50%。结论:本方法操作简便,测定结果准确可靠,为养阴通脑颗粒有效部位黄酮的质量控制提供方法。

Quality control of target area in Yangyin Tongnao Granules by HPLC and UV-VIS

Objective: Determining the contents of general flavone of target area in Yangyin Tongnao Granules with ultraviolet-visible spectrophotometer(UV-VIS).Establish high-performance liquid chromatography(HPLC) to simultaneously detect the contents of puerarin,calycosin and formononetin,which are included in general flavone of target area in Yangyin Tongnao Granules.Methods: Rutin as controls product,determine the contents of general flavone of target area in Yangyin Tongnao Granules by UV-VIS.A HPLC method was set up with the gradient elution mode,using Eclipse XDB-C18,mobile phase was acetonitrile-0.04% phosphoric acid water,flow rate was 1.00mL/min,temperature was 30℃ with the wavelength at 250 nm.Results: The results show,the contents of general flavone of target area in Yangyin Tongnao Granules is above 70 percent.For puerarin,the calibration curve was linear(r=0.9998) in the range of 0.530-296.300mg/L,the average recovery was 99.8%,RSD=0.4%.For calycosin,the calibration curve was linear(r=0.9994) in the range of 0.064-44.440mg/L,the average recovery was 99.70%,RSD=1.10%.For formononetin,the calibration curve was linear(r=0.9994) in the range of 0.064-43.110mg/L,the average recovery was 99.40%,RSD=1.50%.Conclusion: This method is easy to operate and determination result is accurate and reliable,can be used for quality control of Yangyin Tongnao Granules.