| 骨宁颗粒质量标准研究 |
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| 引用本文： | 梅昕,蒋云根,刘婧,白玫.骨宁颗粒质量标准研究[J].武警医学院学报,2014(2):131-133. |
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| 作者姓名： | 梅昕 蒋云根 刘婧 白玫 |
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| 作者单位： | 武警江西总队医院药剂科,江西南昌330001 |
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| 摘 要： | 【目的】建立骨宁颗粒质量标准。【方法】采用薄层色谱法对骨宁颗粒中白芍、葛根、威灵仙进行定性鉴别，并用高效液相色谱法对芍药苷的含量进行测定。色谱柱填充剂：十八烷基硅烷健合硅胶；流动相：乙腈-0．1％磷酸溶液（14：86，v／v）；流速：1．0ml／min；检测波长：230nm；柱温：30℃。【结果】薄层色谱分离清晰，阴性无干扰；芍药苷在0．4028—2．016μg范围内与峰面积有良好线性关系，r=0．9996，芍药苷的平均回收率为99．15％，RSD％=0．90％（n=6）。【结论】该方法专属性强，灵敏度高，重复性好，可用于骨宁颗粒的质量控制。
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| 关 键 词： | 骨宁 质量标准 薄层色谱 高效液相色谱 芍药苷 |
Study on the quality standard of Gu Ning Granules |
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| MEI Xin,JIANG Yun-gen,LIU Jing,BAI Mei.Study on the quality standard of Gu Ning Granules[J].Acta Academiae Medicinae CPAPF,2014(2):131-133. |
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| Authors: | MEI Xin JIANG Yun-gen LIU Jing BAI Mei |
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| Institution: | (Department of Pharmacy, Jiangxi Provincial Corps Hospital of PAPF, Nanchang 330001, China) |
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| Abstract: | Objective]To establish the quality standard for Gu Ning Granules. Methods]Herbaceous peony, pueraria and clematis in Gu Ning Granules were identified by TLC. The content of paeoniflorin was determined by HPLC with C 18 column and mobile phase of acetonitrile-0.1% phosphonic acid （14： 86） under the flow rate of 1.0 ml/min, the column temperature was 30℃ and the detection wavelength was 230 nm. Result] The TLC spots developed were fairly clear, and the blank test showed no interference. Paeoniflorin was linear in the range of 0.4028 - 2.016 μg. The average recovery was 99.15% and RSD was 0.90% （n = 6）. Conclusion ] The method has good specialization, sensitivity and reproducibility, and can be used for quality control of Gu Ning Granules. |
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| Keywords: | Gu Ning Quality standard TLC HPLC Paeoniflorin |
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