阻塞性睡眠呼吸暂停低通气综合征对患者外周血淋巴细胞免疫表型及C反应蛋白的影响

目的:通过分析阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者外周血淋巴细胞免疫表型及C反应蛋白(CRP),探讨OSAHS对机体免疫功能及炎症反应的影响。方法:根据多导睡眠图检测结果,将60例OSAHS患者分为轻度OSAHS组20例,中度OSAHS组22例,重度OSAHS组18例,选取排除OSAHS诊断的健康体检者40名作为对照组。比较受试者的年龄、睡眠状况及睡眠中末梢血氧,抽取空腹血两份,分别采用流式细胞仪行淋巴细胞免疫表型分析,检查血CRP值。结果:OSAHS组最低血氧、平均血氧均较对照组明显下降,且在OSAHS严重程度间差异均有统计学意义(P<0.01)。OSAHS组患者外周血CD3+、CD4+/CD3+及CD4+/CD8+比值均较对照组下降(P<0.05~P<0.01),在OSAHS严重程度间差异均有统计学意义(P<0.01);OSAHS组CD8+/CD3+较对照组升高(P<0.05~P<0.01);OSAHS组CD16+CD56+较对照组升高(P<0.05);CD19+与对照组差异无统计学意义(P>0.05);重度OSAHS组外周血CRP均较对照组和轻度OSAHS组升高(P<0.01和P<0.05)。结论:OSAHS导致机体免疫功能下降,同时伴有炎症反应增强,并且与OSAHS严重程度有一定关系。

The impact of obstructive sleep apnea hypopnea syndrome on the changes of peripheral blood lymphocyte immunophenotype and C-reactive protein

Objective:To explore the impact of obstructive sleep apnea hypopnea syndrome on body immune function and inflammatory reaction by analyzing the changes of peripheral blood lymphocyte immunophenotype and C-reactive protein ( CRP ) in obstructive sleep apnea hypopnea syndrome( OSAHS) patients. Methods:According to the results of polysomnography test,60 subjects diagnosed with obstructive sleep apnea hypopnea syndrome were divided into mild OSAHS group(20 cases),moderate OSAHS group (22 cases) and severe OSAHS group(18 cases),and 40 subjects without OSAHS were selected as control group. The age,sleep patterns and peripheral blood oxygen content during sleeping period of all subjects were compared. Double fasting blood was drawn in each subject which was used to analyze lymphocyte immunophenotype by flow cell meter and check blood CRP values. Results:Average blood oxygen content and lowest blood oxygen content in OSAHS patients were significantly lower than those in controls,there had the statistical significances of the degree of decline of blood oxygen among the different degree OSAHS groups (P<0. 01). Peripheral blood CD3 +,the ratios of CD4 + /CD3 + and CD4 + /CD8 + in OSAHS patients were significantly lower than those in controls(P<0. 05 to P<0.01),there had the statistical significances among the different degree OSAHS groups (P <0. 01). The ratio of CD8 + /CD3 + in OSAHS patients was significantly higher than that in controls(P<0. 05 to P<0. 01). The percentage of CD16 + CD56 + lymphocyte in OSAHS patients was higher than that in controls(P<0. 05). There were no statistical significance of CD19 + lymphocyte percentage between OSAHS patients and the controls(P>0. 05). Peripheral blood CRP value in severe OSAHS patients was increased,compared with that in mild OSAHS patients and the controls(P<0. 01 and P<0. 05). Conclusions:OSAHS decreases immunity function of the body and activates inflammation reaction,which was associated with the severity degree of OSAHS.