水飞蓟素通过抑制心肌细胞凋亡减轻心肌梗死

目的：观察水飞蓟素对心肌梗死小鼠的血流动力学、梗死面积及梗死边缘区凋亡蛋白表达情况。方法：将60只小鼠随机分为心肌梗死组、假手术组、心肌梗死+水飞蓟素组和心肌梗死溶剂组。建模成功4周后检测小鼠血流动力学变化,进行心脏超声检查,评价梗死面积、细胞凋亡指数以及凋亡蛋白Bcl-2、Bax、Cleaved-Caspase3的表达。结果：与心肌梗死组小鼠相比,水飞蓟素可显著减轻心肌梗死,改善心梗小鼠心功能,降低心肌细胞凋亡指数,增强Bcl-2蛋白表达和减弱Bax和Cleaved-Caspase3蛋白表达。结论：水飞蓟素能够减轻心肌梗死,改善心梗小鼠心室收缩功能,保护心肌,减少心肌细胞的凋亡,其机制与升高Bcl-2蛋白、降低Bax和Cleaved-Caspase3蛋白表达水平有关。

Silymarin alleviates myocardial infarction by inhibiting myocardial cell apoptosis

Objective To evaluate the cardioprotective effects of silymarin on mice with acute myocardial infarction (AMI) and its possible mechanism. Methods A total of 60 male C57BL/6 mice were randomly divided into 4 groups: Sham group, AMI group, AMI+Silymarin group, and AMI+Vehicle group. Drug administration was started at the second day after modeling and lasted for four weeks. Four weeks after modeling, hemodynamic parameters and quantitative echocardiographic assessments were obtained to evaluate the cardiac function. Myocardium infarct area was estimated by H-E staining. Cell apoptosis was observed by TUNEL and apoptotic index was calculated. Protein expressions of Bcl-2, Bax and Cleaved Caspase-3 were detected by Western blotting analysis. Results Compared with AMI group, AMI+Silymarin group had improved hemodynamic parameters and cardiac function, significantly reduced infarction area and histopathology changes of the infarcted area (P<0.05), significantly decreased cardiomyocyte apoptotic index (P<0.05), significantly increased protein expression of Bcl-2 and significantly decreased expression of Bax and Cleaved Caspase-3 (P<0.05). Conclusion Silymarin can reduce infarction area and improve cardiac function in mice, which might be related to inhibition of the myocardial apoptosis.