氯氰菊酯对雄性小鼠肝脏组织的氧化损伤

目的:研究氯氰菊酯(cypermethrin,CYP)对雄性小鼠肝脏组织的氧化损伤。方法:将40只昆明种雄性小鼠随机均分为双蒸水灌胃(阴性对照组)和CYP 5、10和20 mg/kg染毒组,连续3周经口灌胃染毒。观察小鼠肝脏脏器系数,同时检测小鼠肝组织中谷胱甘肽过氧化物酶、过氧化氢酶、总超氧化物歧化酶活性及丙二醛含量。结果:随着CYP染毒剂量的升高,CYP 5 mg/kg和10 mg/kg组小鼠肝脏脏器系数均高于阴性对照组(P<0.01和P<0.05);CYP 10 mg/kg和20 mg/kg染毒组小鼠肝脏组织中谷胱甘肽过氧化物酶与CYP 20 mg/kg组过氧化氢酶活性均低于阴性对照组(P<0.01),CYP 5 mg/kg和10 mg/kg组总超氧化物歧化酶活性均高于阴性对照组(P<0.01),CYP 20 mg/kg组小鼠丙二醛含量亦显著高于阴性对照组(P<0.01)。结论:CYP可以引起雄性小鼠肝脏组织的氧化损伤,且损伤程度与CYP剂量有关。

The oxidative injury induced by cypermethrin in liver tissue of male mice

Objective:To study the oxidative injury induced by cypermethrin(CYP) in liver tissue of male mice. Methods:Forty Kunming male mice were randomly divided into the double steaming water lavage group( negative control group) and CYP at different dosage groups,the exposure dosages of CYP were 5,10 and 20 mg/kg respectively. CYP was administered to mice by gavage once daily for three weeks, then the liver index, the activities of glutathione peroxidase, catalase and total superoxide dismutase, the content of malondialdehyde in mice liver tissue were measured. Results:With the increase of CYP dosage,in the 5 mg/kg and 10 mg/kg CYP groups,the mice liver index was higher than that in the negative control group(P<0. 01 and P<0. 05);the activities of glutathione peroxidase in 10 mg/kg and 20 mg/kg CYP groups and catalase in the 20 mg/kg CYP group were decreased compared with the negative control group (P<0. 01),the activities of superoxide dismutase were decreased in 5 mg/kg and 10 mg/kg CYP groups (P<0. 01). Malondialdehyde content was increased in the 20 mg/kg CYP group compared with the negative control group ( P < 0. 01 ). Conclusions:Cypermethrin can induce the oxidative damage in male mice liver tissue.