癌性胸水p16基因纯合性缺失检测的临床意义

研究癌性胸水Ｐ１６基因纯合性缺失检测的临床意义。方法：应用ＰＣＲ技术检测胸水Ｐ１６基因第一、二外显子纯合性缺失，并结合胸水脱落细胞学检测分析其在临床诊断中的意义。结果表明所检３１例肺癌所致癌性胸水标本中均无出现Ｐ１６基因第一外显子纯合性缺失，１２例有Ｐ１６基因第二外显子纯合性缺失，阳性率为３８．７１％。

The clinical significance of deletion of p16 gene homozygous deletion in malignant pleural effusion

Objective:To investigate the clinical significance ofhomozygous deletion of p16 gene in malignant pleural effusionMethods:The homozygous deletion of p16 gene exon1 and exon2 was examined by polymerase chain reaction (PCR) in 31 specimens of pleural effusions from lung cancer patients and compared with the determination of exfoliated cell in same specimens fromwhich the clinical value was analysed in clinical diagnosisResults:No homozygous deletion of p16 gene exon1 was found in 31 malignant pleural effusion samplesThe homozygous deletion of p16 gene exon2 was detected in 12 sampls from31 pleural effusions, while cytology positivity was found in 16 of all examined pleural effusionsThe positive rate in 31 malignant pleural effusion samplesby the determination of homozygous deletion of p16 gene and the examination of exfoliated cell was 3871%(12/31) and 5162%(16/31), respectivelyThe positive rate could be enhanced from 5162(16/31) to 7097%(22/31) by using both ofabove methods, There was no significnt difference of positive rate between homozygous deletion of p16 gene exon2 and exfoliated cytology The overall positive rate of both examination was higher than that of only the determination of homozygous deletion of p16 gene exon2 or exfoliated cytology in malignant pleural effusionNo homozygous deletion of p16 gene was found in 21 tuberculosis pleural effusion samplesConclusions:Our present data suggest that combining examinations of exfoliated cytology and homozygous deletion of p16 gene exon2 in pleural effusion can not only increase the positive rate of diagnosis, but also recruit and enhance the diagnostic value of conventional pleural cytology