体外产气法对苏丹草与棉秆间组合效应的研究

为了探讨苏丹草(Sudan Grass,SG)与棉秆(Cotton Straw,CS)在瘤胃发酵中的组合效应,本研究将苏丹草与棉秆以100∶0、75∶25、50∶50、25∶75、0∶100比例进行组合,每个组合3个重复,利用瘤胃液体外发酵技术,分析GP(产气量)、pH值、瘤胃液NH3-N(氨态氮)和VFA(挥发性脂肪酸)含量,评价产气量组合效应以及综合组合效应。结果表明:(1)苏丹草和棉秆比例为50∶50和25∶75时,产气量与产气速率较高。(2)24~72h阶段50∶50和25∶75组的组合效应均为正值,且25∶75组的组合效应值最高。(3)苏丹草与棉秆不同比例的组合中,25∶75的组合产生的组合效应最高。

Study on Combination Effect of Sultanate Grass and Cotton Stalk by Gas Production in Vitro

In order to explore the combined effect of Sudan Grass(SG) and Cotton Straw(CS) in rumen fermentation, this study combines Sudan Grass and cotton stalk at the ratio of 100:0, 75:25, 50:50, 25:75 and0:100. Each combination has three repeats.Gas production(GP), pH value, ammonia nitrogen(NH3-N) and Volatile fatty acid(VFA) are analyzed by in vitro fermentation of rumen fluid. The results showed that:(1)When the combination of SG and CS was 50:50 and 25:75, the volume of production and gas production rate were higher than others.(2) The positive combination effect of 50:50 and 25:75 at 24 h-72 h was better, and the positive combination effect of 25:75 was higher than that of the former two. The difference of the positive combination effect between the three proportions was significant(P < 0.05).(3) Among the combinations with different proportion of SG and CS, combination effect of the combination of 25:75 were hightiest.