人大肠癌中Ⅳ型胶原酶与基底膜免疫组织化学及双重染色研究

观察Ⅳ型胶原酶和基底膜在大肠癌生长中的相互关系及Ⅳ型胶原酶的表达与ｐ２１的表达之间的关系。方法：应有和免疫组化双标记方法,对８６例人大肠癌组织的Ⅳ型胶原酶表达及基底膜的改变作对照研究。结果 大肠癌组织中Ⅳ型胶原酶的阳性率为８３．７２％（７３／８６）,与对照组癌周正常肠粘膜的阳性率１０％差异有非常显著意义（Ｐ〈０．０１）,同时,双染色显示Ⅳ型胶原酶阳性的癌组织基底膜表现为不连续,出现缺口,片段缺失或

An immunohistochemical double labelling study on type IV collagenase and basement membrane in human colonic carcinoma

OBJECTIVE: To investigate the relationship between type IV collagenase and basement membrane (BM) and the relationship between type IV collagenase and p21 expression. METHODS: Immunohistochemical double labelling technique was used to study paraffin embedded sections of 86 cases of colon carcinoma. RESULTS: The positive rate of type IV collagenase was 83.72% in carcinoma cases and 10% in normal colorectal mucosa (P < 0.01). Intact BM was seen in normal colorectal mucosa while BM was disrupted and fragmentary in carcinoma cases. A close positive correlation was also found between the expression of type IV collagenase and p21 in colonic carcinoma (r = 0.974, P < 0.01). CONCLUSIONS: The results suggest that type IV collagenase plays an important role in the destruction of BM and invasion by colonic carcinoma.