慢性间断性缺氧后细胞色素C和caspase-3的表蛳达及维生素E和左旋丁基苯酞对其变化的影响

目的 探讨慢性间断性缺氧后细胞色素C的释放、 caspase-3 的激活以及维生素 E 和左旋丁基苯酞(L-NBP)对这些变化的影响。 方法 建立大鼠慢性间断性缺氧模型,并给予 L-NBP 和维生素E 。用 W estern blot方法和免疫组化方法分别检测大鼠皮层的细胞色素 C 释放和 caspase-3 的激活。 结果 对照组可检测到微弱的caspase-3 阳性染色。缺氧组 caspase蛳3 阳性染色显著增加(P < 0.01)。与缺氧组相比,大、小剂量维生素 E 组,大、小剂量L-NBP 组阳性细胞均明显减少( < 0.01)。对照组线粒体可见明显的细胞色素 C 蛋白条带,而胞浆则无此条 P带。缺氧组胞浆可见该蛋白表达, 而线粒体蛋白条带的表达则相应降低。与缺氧组相比大 小剂量维生素 以及 E大、小剂量 L-NBP 均可明显抑制线粒体细胞色素 C 的释放, 有非常显著性差异( P < 0.01)。 结论 慢性间断性缺氧能够诱导细胞色素C由线粒体至胞浆的释放和caspase-3 的激活。维生素 E 和 L-NBP 能够降低细胞色素C的释放及 caspase-3 的裂解激活。

Effects of vitamin E and L-3-n-butylphathalide on the release of cytochromeC and activation of caspase-3 induced by chronic episodic hypoxia

Objective To study cytochrome C release and caspase-3 activation induced by chronic episodic hypoxia (EHYP) and to examine whether Vit E and L-NBP can alleviate these changes. Methods After animal models of EHYP were established, we studied the release of cytochrome C and the activation of caspase-3 in cerebral cortex. Results Caspase-3-immunoreactive(IR) neurons increased significantly in EHYP- exposed rats compared with that of controlled rats(P < 0.01). After having been treated with VitE and L-NBP, caspase-3-IR neurons in all four groups decreased significantly(P<0.01). The expression of cytochrome C protein was present in mitochondrial fractions of brains from controlled rats, no band was seen in the cytosolic fractions. The immunoreactivity of the mitochondrial fractions was decreased in hypoxic brain with a corresponding increase in cytosolic fraction .The expression of cytochrome C protein was significantly inhibited in all treatment groups(P < 0.01). Conclusions EHYP can induce the release of cytochrome C and the activation of caspase-3. Both L-NBP and VitE can ameliorate the release of cytochrome C and the activation of caspase-3.