肝细胞肝癌组织尿激酶型纤溶酶原激活物及其受体的表达与意义

目的:探讨肝细胞肝癌组织中尿激酶型纤溶酶原激活物(uPA)及其受体(uPAR)蛋白和uPAmRNA、uPARmRNA的表达和作用.方法:应用免疫组织化学和RT-PCR方法测定71例肝组织标本,包括对照组(C)12例,肝细胞肝癌组(HCC)34例和癌旁组织组(PTT)25例的uPA、uPAR及其基因的表达,对比分析其与肝癌的临床病理因素的关系.结果:HCC组uPA、uPAR蛋白的平均吸光度值和uPAmRNA、uPARmRNA条带的光密度比值均明显高于C组和PTT组,差异非常显著(P＜0.01),PTT组与C组间差异均无显著性(P＞0.05).uPA、uPAR在包膜不完整,有肝内转移和有肝外转移肝癌中的表达均明显高于包膜完整,无肝内转移和无肝外转移者(P＜0.05或P＜0.01);与肿瘤的大小无关(P＞0.05).结论:肝癌组织uPA、uPAR蛋白及其基因均呈现高表达,可能在肝细胞肝癌的发生、发展,尤其是在侵袭和转移过程中发挥重要的作用.

Expression of urokinase-type plasminogen activator(uPA),its receptor (uPAR) and their mRNA in hepatic carcinoma and significance

Objective: To explore the expression of urokinase-type plasminogen activator,(uPA),its receptor(uPAR,)their mRNA in hepatic carcinoma,its effects and mechanism.Methods: The expression of uPA and uPAR with their mRNA in 71 specimens of human hepatic tissue,including C 12,HCC 34 and PTT 25 were detected with immunohistochemistry and RT-PCR.The expression of uPA and uPAR and its mRNA correlating with clinical and pathological significance were compared and analysed.Results: The uPA and uPAR protein with their mRNA in HCC were significantly higher than that in C and in PTT(all P<0.01);there was significant difference between PTT and C(all P>0.05).Compared by the expression of uPA and uPAR protein with their mRNA in HCC with capsule invaded,intrahepatic and extrahepatic metastasis,there were significant higher than those integral capsule,without intrahe-patic and without extrahepatic metastasis(P<0.05 or P<0.01,)these expression was not significant correlation with the magnitude of HCC(all P>0.05),with lower of Edmondson grade,they were higher stepup,but were not statistically significant.Conclusion: The uPA and uPAR show strongly expression in HCC and they may play significant roles in the generation,development,invasion and metastasis of HCC.