灵芝多糖对2型糖尿病大鼠血流动力学及氧化应激的影响

目的:探讨灵芝多糖(GLPs)对2型糖尿病大鼠血流动力学及心肌组织中NO,SOD,MDA,CAT,GSH-Px含量的影响.方法:SD大鼠高脂饮食喂养4周后,腹腔注射链脲佐菌素(STZ)30 mg·kg~(-1)建立2型糖尿病(T2DM)模型.成模后将大鼠随机分为6组:正常对照组、模型对照组、灵芝多糖低、中、高剂量治疗组(200,400,800 mg·kg~(-1))、小檗碱阳性对照组,给予药物治疗,第16周末测定血流动力学参数(LVSP,LVEDP,dp/dt_(max),-dp/dt_(max)),检测心脏NO,SOD,MDA,CAT,GSH-Px水平;用电镜观察左室心肌组织超微结构.结果:与模型对照组比较,灵芝多糖中、高剂量能有效降低血糖水平;改善糖尿病大鼠血流动力学指标:降低LVEP,升高LVSP,dp/dt_(max),-dp/dt_(max);能够有效对抗氧化应激作用.同时可以显著改善心肌超微结构.其中高剂量组效果优于中剂量组和BerG.结论:灵芝多糖能够明显改善T2DM大鼠血流动力学参数,降低其氧化应激的水平.

Effect of Ganoderma lucidum polysaccharides on hemodynamic and antioxidation in T2DM rats

Objective: To investigate the effect of Ganoderma lucidum polysaccharides(GLPs) on hemodynamic and antioxidation in the T2DM rats. Method: SD rats were fed high-fat diet for 4 weeks and then were injected STZ (30 mg·kg^-1) to induce the type 2 diabetes mellitus (T2DM). Once the T2DM models were set successfully, rats were randomized into six groups: normal group (NG),group of diabetes mellitus (DMG),groups of low dosage (GLPs-LG),middle dosage (GLPs-MG),high dosage (GLPs-HG) and berberine (BerG). They received GLPs with different dosages (200, 400, 800 mg·kg^-1) and berberine (30 mg·kg^-1) continually for 16 weeks. At 16th weekend, the following indices of rats were measured respectively: blood glucose, hemodynamic including LVSP, LVEDP, dp/dt_max and -dp/dt_max and the contents of NO, SOD, MDA, GSH-Px, CAT in cardiac tissue. Besides, myocardial ultrastructure was observed by electron microscope. Result: Both the middle dosage and the high dosage of GLPs could low blood glucose effectively, and they could reduce LVEP but increase -dp/dt_max. Meanwhile, they could activate GSH-Px, CAT, SOD, NO, but reduce MDA in cardiac tissue and improve the myocardial ultrastructure. Compared to the DM group, the middle dosage, high dosage of GLPs and berberine showed significant improvement. Compared to the berberine group, the middle dosage showed the same effect, but the high dosage was more effective than berberine. Conclusion: There is a confirmed action of GLPs in improving the hemodynamic and antioxidation in cardiac tissue of T2DM rats.