仙仲注射液对兔早期膝骨性关节炎形态学及胰岛素样生长因子-1的影响

目的评价仙仲注射液对兔早期膝骨性关节炎形态学及胰岛素样生长因子-1（IGF-1）的影响。方法120只大耳白兔随机分为空白组、模型组（生理盐水每周0．5mL，1次）、阳性药组（海诺特每周0．5mL，1次）、仙仲Ⅰ、Ⅱ组（仙仲注射液每周0．5mL，1次和2次），均为关节腔给药。造模8周后观察膝关节功能变化，并采集动物血清、关节液进行IGF-1检测；将关节软骨标本进行Mallory染色观察组织形态。结果（1）关节功能：与模型组比较仙仲Ⅰ关节功能显著改善（P〈0．01），仙仲Ⅰ组效果与阳性药组相当。（2）关节软骨形态学变化：Mallory染色见阳性药组和仙仲Ⅰ组全层未钙化，软骨蓝绿色胶原保存较完整。（3）关节液、血清中IGF-1：阳性药组及仙仲Ⅰ、Ⅱ组均升高（P〈0．05）。结论仙仲注射液对于早期膝骨性关节炎的关节功能有一定改善，并对软骨退变有修护作用。

Study on Effect of Xianzhong Injection on Morphology of Knee and Insulin-like Growth Factor-1 in Rabbits with Early Stage Osteoarthritis

OBJECTIVE: To investigate the effect of Xianzhong Injection (XI) on morphology of knee and insulin-like growth factor-1 (IGF-1) in rabbits with early stage osteoarthritis (OA). METHODS: One hundred and twenty rabbits were randomly divided into 5 groups, the blank group, the model group treated with 0.5 mL normal saline once a week, the positive control group treated with 0.5 mL hyaluronate once a week, and the XI 1 group and XI 2 group with 0.5 mL XI once and twice a week, respectively, all the medicines were medicated via intra-articular injection. Knee joint function was examined 8 weeks after modeling, IGF-1 content in serum and synovia was detected, and the histologic examination of articular cartilage was observed by Mallory staining as well. RESULTS: The improvement of knee joint function was better in the XI 1 and 2 groups than that in the model group (P < 0.01), and that in the XI 1 group was equivalent to that in the positive control group (P > 0.05). Histologic examination showed there was no calcification in all the layers of cartilage in the positive control group and the XI 1 group with complete cartilaginous bluish-green collagen preserved. The content of IGF-1 increased in synovia and serum in the positive control group and the two XI groups as compared with that in the model group (P < 0.05). CONCLUSION: XI can improve knee joint function to certain extent in early OA, and repair the degenerative cartilage.