非小细胞肺癌组织FHIT和突变型P53表达的关联性及相互作用

目的:探讨FHIT和突变型P53在非小细胞肺癌(NSCLC)中表达的相关性以及两者潜在的相互作用. 方法: 采用免疫组化PicTureTM通用型二步法,检测FHIT和突变型P53蛋白在63例NSCLC和12例肺良性病变组织中的表达. 结果: NSCLC组织FHIT阳性率(42.9%)低于肺良性病变组织(75.0%);FHIT表达与病理分级(r=0.358, P＜0.05), TNM分期(r=0.314, P＜0.05)及淋巴结转移(r=0.380, P＜0.05)相关. 突变型P53在NSCLC组织中阳性率(52.4%)高于正常肺组织(8.3%);突变型P53表达与TNM分期(r=0.391, P＜0.05)及淋巴结转移(r=0.250, P＜0.05)相关,与病理分级无关联性. FHIT与突变型P53蛋白在63例NSCLC中表达相关(r=0.258, P＜0.05). 结论: P53突变和FHIT表达下调对NSCLC发生、发展均可能起重要作用, 在其病变过程中可能同时存在两条途径, 并可能相互交叉.

Correlation and potential interaction between FHIT and mutant P53 expression in non-small cell lung cancer

AIM: To investigate the correlation and potential interaction between FHIT and mutant P53 expression in non-small cell lung cancer (NSCLC). METHODS: The expressions of FHIT and mutant P53 in 63 specimens of NSCLC and 12 cases of benign lung lesion tissues (as controls) were examined by immunohistochemical technique. RESULTS: The positive rate of FHIT in NSCLC (42.9%) was significantly lower than that in the controls (75.0%); the expression of FHIT was significantly correlated with pathological grade (r=0.358, P<0.05), TNM stage (r=0.314, P<0.05) and lymph node metastasis (r=0.380, P<0.05). The positive rate of mutant P53 in NSCLC (52.4%) was significantly higher than that in the controls (8.3%); the expression of mutant P53 was significantly related to TNM stage (r=0.391, P<0.05) and lymph node metastasis (r=0.250, P<0.05), but not to pathological grade. The expressions of FHIT and mutant P53 in NSCLC were significantly correlated (r=0.258, P<0.05). CONCLUSION: The down-regulation of FHIT and mutant P53 possibly play important roles in the development of NSCLC.