孕酮和IL-15对人早孕蜕膜子宫自然杀伤(uNK)细胞促血管生成因子的调节

目的:探讨孕酮和IL-15对蜕膜子宫自然杀伤细胞(uNK)在早孕蜕膜血管生成/重塑过程中的作用。方法:免疫磁珠(MACS)分离及纯化蜕膜uNK细胞后进行体外培养,分别用不同浓度孕酮或IL-15干预72h后获取培养上清液和细胞。采用RT-PCR和ELISA检测蜕膜uNK细胞的VEGF-A、VEGF-C、Ang2的mRNA转录和蛋白表达。结果:蜕膜uNK细胞可表达多种与血管形成有关的生物活性分子,与未干预的空白对照组相比,IL-15促进uNK细胞表达VEGF-A、VEGF-C(P<0.05),且呈剂量依赖关系,但对Ang2无影响;而孕酮对uNK细胞表达上述因子均无显著影响(P>0.05)。结论:妊娠早期蜕膜uNK细胞通过表达多种促血管生成因子,在早孕蜕膜血管生成/重塑中起着重要的作用,IL-15对此有直接促进作用。

Effect of Progesterone and Interleukin-15 on Angiogenic Growth Factor of the Decidual Uterine Natural Killer Cells in Early Pregnancy

Objective: To explore the effects of progesterone(P) and interleukin-15(IL-15) on decidual uterine natural killer cell (uNK cell) of early pregnancy. Methods: CD56-microbeads (MACS) were used to isolate and purify uNK cells from human decidua of normal early pregnancy, and then cultured in vitro. These uNK cells were treated with P or IL-15 in different concentrations at least three holes for each concentration. After treated 72 h, ELISA and RT-PCR were used to detect the expression of VEGF-A, VEGF-C, and Ang2 in these uNK cells on their supernatant. Results: These decidual uNK cells expressed multi-angiogenic growth factors. IL-15 enhanced the gene or protein expression of VEGF-A/VEGF-C of decidual uNK cells in a dose-dependent manners (P<0.05), but no impact on Ang2 expression. However, P had no effect on gene expression of these angiogenic growth factors in decidual uNK cells, neither in protein level (P>0.05). Conclusion: uNK cells play an important role in the angiogenesis of human decidua in early pregnancy, through expressing multiple angiogenic growth factors, which are promoted by IL-15 directly.