Transfer of Neurospora kalilo plasmids among species and strains by introgression |
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Authors: | Jin-Woo Bok Cynthia He Anthony J F Griffiths |
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Institution: | (1) Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, B.C. V6T 1Z4, Canada e-mail: agriff@unixg.ubc.ca, CA |
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Abstract: | There are four different variants of the kalilo “family” of linear mitochondrial plasmids. This family is found in several
heterothallic species and one pseudohomothallic species of Neurospora, as well as in one homothallic species of Gelasinospora. The mode of dispersal of these plasmids is not known. Horizontal transmission has proved difficult to demonstrate. Another
possibility is transfer by introgression, and this is modelled in the present paper. We have used introgression and subsequent
heterokaryosis to successfully transfer the LA-kalilo plasmid from a Haitian strain of Neurospora crassa to the standard Oak Ridge N. crassa background, the LA-kalilo plasmid from the pseudohomothallic Neurospora tetrasperma to N. crassa, and the kalilo plasmid from N. crassa to N. tetrasperma. Thus, introgression is shown to be a possible avenue of dispersal between species. The recipient strains were all senescent
but the mechanism of this senescence is not known. It could be caused by the plasmids, but if so the mechanism is novel since
plasmid/mtDNA junction fragments of the type found in the standard mode of mtDNA insertion could not be detected. However,
mtDNA changes were observed in the senescent recipients.
Received: 15 February / 24 June 1999 |
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Keywords: | Neurospora Kalilo Plasmid mtDNA Heterokaryon |
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