市售沙棘HPLC指纹图谱一致性及抗氧化活性谱效关系

目的:比较市售沙棘HPLC指纹图谱的差异,并研究其HPLC指纹图谱与抗氧化活性的相关性,为探寻沙棘抗氧化物质基础及饮片质量评价提供参考。方法:HPLC建立不同来源沙棘饮片指纹图谱及相似度,系统聚类分析其差异;1,1-二苯基-2-三硝基苯肼(DPPH)法研究其抗氧化作用,灰色关联度分析法研究谱效关系。结果:建立了14批沙棘HPLC指纹图谱,确定了其中10个色谱峰为共有峰,其中10号峰为槲皮素,各样品聚为两类,相似度在0.862~0.992;14批沙棘饮片均有不同程度的清除自由基作用;10个共有峰与清除自由基的关联度在0.758~0.662,大小依次为x10x3x7x4x5x9x1x6x8x2,其中10号峰槲皮素与清除DPPH自由基作用关联度最大。结论:市售沙棘饮片总体质量比较一致,但不同地区间的质量有一定的差异,沙棘抗氧化作用为"多成分"共同起效的结果。通过HPLC指纹图谱一致性研究及"谱-效"关系分析探寻其抗氧化物质基础,可为沙棘饮片质量评价提供依据。

Correlation Analysis Between HPLC Fingerprint of Hippophae Fructus Pieces and Antioxidant Activity

Objective: To compare the difference of HPLC fingerprints of commercially available Hippophae Fructus pieces, explore the correlation between their HPLC fingerprints and antioxidant activity, and provide reference for exploring the antioxidant material basis of Hippophae Fructus as well as quality evaluation. Method: HPLC fingerprints of different sources of Hippophae Fructus pieces were established, and the difference were analyzed by the similarity and system cluster analysis method;antioxidant effect was determined by 1, 1 diphenyl-2-trinitrobenzene hydrazine (DPPH) method, and the spectrum-effect relationship was studied by gray correlation analysis method. Result: The HPLC fingerprints of 14 batches of Hippophae Fructus were established;10 common peaks were identified, and No. 10 peak was the chromatographic peak for quercetin;the samples came into two categories, with the similarity of 0.862-0.992;the 14 batches of Hippophae Fructus pieces had different levels of free radical scavenging effect;the correlation between 10 common peaks and free radical scavenging was in the range of 0.758-0.662, with an order of x10 > x3 > x7 > x4 > x5 > x9 > x1 > x6 > x8 > x2 from high to low, where No. 10 peak for quercetin had the maximum correlation with DPPH free radical scavenging effect. Conclusion: The quality of commercially available Hippophae Fructus was relatively consistent, but there were some differences in quality between those from different regions, and their antioxidation effect was resulted from the common onset of ''multi-components''. By exploring the antioxidant material basis of Hippophae Fructus through the study of HPLC fingerprints consistency and spectrum-effect relationship analysis, it can provide reference for quality evaluation of Hippophae Fructus.