| 基因探针法快速检测食品中金黄色葡萄球菌的研究 |
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| 引用本文： | 陈彬,黄晓蓉,汤敏英,郑晶,林杰. 基因探针法快速检测食品中金黄色葡萄球菌的研究[J]. 现代预防医学, 2007, 34(6): 1017-1018,1021 |
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| 作者姓名： | 陈彬 黄晓蓉 汤敏英 郑晶 林杰 |
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| 作者单位： | 福建出入境检验检疫局,福州,350003 |
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| 摘 要： | [目的]应用Accuprobe基因探针方法快速检测食品中金黄色葡萄球菌（Staphylococcus aureus）。[方法]本实验针对基因探针法检测金葡菌的特异性、敏感性、准确性进行研究，比较该方法与传统国标法的检测结果。[结果]基因探针方法检测食品中金葡菌特异性强，采用增菌肉汤的检测低限为107cUfnJ，检测金葡菌的结果与国标法相一致；对食品中金葡菌鉴定时间仅需30min，简便快捷。[结论]基因探针方法可用于食品中金葡菌的快速检测。
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| 关 键 词： | 金黄色葡萄球菌 基因探针 检测 |
| 文章编号： | 1003-8507（2007）06-1017-03 |
| 收稿时间： | 2006-04-23 |
| 修稿时间： | 2006-04-23 |
STUDY ON THE RAPID DETECTION OF STAPHYLOCOCCUS AUREUS WITH GENE PROBE METHOD IN FOODSTUFFS |
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| CHEN Bin, HUANG Xiao-rong, TANG Min-ying,et al.. STUDY ON THE RAPID DETECTION OF STAPHYLOCOCCUS AUREUS WITH GENE PROBE METHOD IN FOODSTUFFS[J]. Modern Preventive Medicine, 2007, 34(6): 1017-1018,1021 |
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| Authors: | CHEN Bin HUANG Xiao-rong TANG Min-ying et al. |
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| Affiliation: | Fujian Entry- Exit Inspection and Quarantine Bureau, Fuzhou 350001, China |
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| Abstract: | [Objective] A rapid detection of Staphylococcus aureus in foodstuffs by Accuprobe gene probe method was studied. [Methods] The detection results of Staphylococcus aureus with gene probe method in specificity, sensitivity, veracity was studied and compared with Chinese national GB standard method. [ Results] Gene probe method was a high-specificity detection method for Staphylococcus aureus and its low limit of detecion was 107 cfu/ml. There was no significant difference in results by these two methods. The whole confirmation process could be finished within 30 min. [ Conclusions] Accuprobe gene method was a rapid and convenient method for detection of Staphylococcus aureus in foodstuffs and the result is accurate and reliable. |
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| Keywords: | Staphylococcus aureus Gene probe method Detection |
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